摘要
目的探讨不同浓度葡萄糖溶液对白纹伊蚊和淡色库蚊的蚊幼存活率,以及诱蚊、诱卵效果的影响,为基于糖饵剂的灭蚊技术研发提供支持。方法取白瓷碗分别加入100 mL 3%、5%、8%、10%和15%的葡萄糖溶液,每碗投放10只白纹伊蚊或淡色库蚊Ⅳ龄蚊幼,第2、4、6、24、48、72 h观察蚊幼存活情况。饲养笼内投放饱血白纹伊蚊和淡色库蚊雌蚊各50只,四角各放置盛有5%、8%和15%葡萄糖溶液的产卵杯各1个,观察72 h总产卵数。在模拟房内投放饱血和饥饿白纹伊蚊、淡色库蚊雌蚊各100只,盛有5%和8%浓度葡萄糖溶液和粘虫板制成的黑色简易灭蚊桶各1个,观察6 d后成蚊数和蚊卵数。以上试验均重复3次,以去氯自来水为对照。结果随着葡萄糖溶液浓度增加,白纹伊蚊和淡色库蚊蚊幼的72 h矫正死亡率升高,高于5%的葡萄糖溶液不适宜蚊幼存活。诱卵结果显示,随着葡萄糖溶液浓度增加,对白蚊伊蚊诱卵效果下降;5%和8%葡萄糖溶液组诱卵效果相似,白纹伊蚊卵平均数分别为686.67枚和682.33枚,淡色库蚊卵筏平均数分别为3.00块和2.33块。模拟房内,对照组、5%和8%葡萄糖溶液组诱捕成蚊平均数分别为93.33、105.00和130.33只,8%葡萄糖溶液组诱蚊效果强于对照组(F=3.283,P=0.030);白纹伊蚊蚊卵平均数(蚊卵数+蚊幼数)分别为70.33、55.33和63.00只,差异有统计学意义(H=6.761,P=0.034)。结论5%及以上浓度的葡萄糖溶液可有效抑制白纹伊蚊和淡色库蚊蚊幼存活,且具备较好的诱蚊效果及一定的诱卵效果。
Objective To explore the effects of different concentrations of glucose solution on the survival of Aedes albopictus and Culex pipiens pallens larvae,the attraction to mosquitoes and egg-laying behaviors,so as to provide the reference for developing mosquito control technology based on sugar bait.Methods White porcelain bowls were filled with 100 mL of 3%,5%,8%,10%and 15%glucose solutions.Ten of fourth instar larvae of Aedes albopictus or Culex pipiens pallens were added to each bowl,and the survival of larvae was recorded after 2,4,6,24,48 and 72 hours.Egg-laying cups containing 5%,8%and 15%glucose solution were put in mosquito cages containing fully blooded female mosquitoes of Aedes albopictus and Culex pipiens pallens(50 mosquitoes each),and the total number of eggs laid in 72 hours was observed.The analogous site room was filled with fully blooded and starved female mosquitoes of Aedes albopictus and Culex pipiens pallens(100 mosquitoes each),and simple mosquito control buckets containing 5%and 8%glucose solution and black sticky insect plates.The number of mosquitoes and eggs was observed after 6 days.All the above experiments were repeated 3 times using dechlorinated water as the control.Results The 72 hour corrected mortality rates of Aedes albopictus and Culex pipiens pallens larvae gradually increased with the increase of glucose concentration.The glucose solution with 5%and higher concentrations was not suitable for mosquito larvae to survive.The attraction of egg-laying behaviors to Aedes albopictus and Culex pipiens pallens gradually decreased with the increase of glucose concentration.The effects were similar between 5%and 8%glucose solution,with the averages of 686.67 and 682.33 eggs for Aedes albopictus,and 3.00 and 2.33 egg rafts for Culex pipiens pallens.In analogous site room,there were 93.33,105.00 and 130.33 adult mosquitoes captured on average in the control group,5%and 8%glucose solution groups,respectively,with 8%glucose solution group more attractive to adult mosquitoes than the control g
作者
吴瑜燕
陈恩富
刘钦梅
李天奇
王金娜
罗明宇
龚震宇
WU Yuyan;CHEN Enfu;LIU Qinmei;LI Tianqi;WANG Jinna;LUO Mingyu;GONG Zhenyu(Department of Infectious Disease Control and Prevention,Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou,Zhejiang 310051,China)
出处
《预防医学》
2024年第6期543-547,共5页
CHINA PREVENTIVE MEDICINE JOURNAL
基金
2022年度“尖兵”“领雁”研发攻关计划项目(2022C03109)
2022年度浙江省卫生健康科技计划项目(2022KY720)
2024年度浙江省医药卫生科技计划项目(2024KY893)。
关键词
糖饵剂
白纹伊蚊
淡色库蚊
蚊虫防制
sugar bait
Aedes albopictus
Culex pipiens pallens
mosquito control
