摘要
目的 通过RiboPrinter全自动微生物基因指纹鉴定系统对中药片剂待压片粉污染的耐胆盐阴性菌进行溯源分析,找到污染源。方法 用VITEK2 Compact全自动微生物鉴定系统、RiboPrinter全自动微生物基因指纹鉴定系统、16SrRNA(16S核糖体RNA)序列分析、MALDI-TOF MS(基质辅助激光解吸电离飞行时间质谱)对待压片粉中和环境中分离的16株耐胆盐革兰氏阴性菌进行鉴定并比较结果差异,再采用RiboPrinter方法对鉴定结果一致的菌株进行基因指纹图谱比对、溯源,并利用BioNumerics软件进行聚类分析。结果 待压片粉中分离的6株菌株与沸腾干燥机布袋上分离的1株菌株鉴定结果一致,为热生泛菌(Pantoea calida),通过比较,MALDI-TOF MS鉴定速度更快,分辨率更高。RiboPrinter溯源分析表明7株热生泛菌同源性极高,相似度为0.94±0.03。结论 通过RiboPrinter溯源结果和BioNumerics构建树状图分析,推断待压片粉污染来自沸腾干燥机布袋,分析污染风险高低,并提出污染解决措施和方案。
Objective To trace and analyze the source of contamination of bile salt resistant gram-negative bacteria in the powder of traditional Chinese medicine tablets through RiboPrinter method.Methods Totally 16 strains of bile salt resistant gram-negative bacteria isolated from the powder of traditional Chinese medicine tablets and the environment were identified and compared with VITEK2 Compact,RiboPrinter,16SrRNA,and MALDI-TOF MS methods.Finally,the RiboPrinter method was used to trace the gene fingerprints of the strains with consistent identification results.Results The identification of the 6 strains from the powder of traditional Chinese medicine tablets was consistent with 1 strain from the boiling dryer cloth bag,namely Pantoea calida.Through comparison,MALDI-TOF MS had a faster identification speed and higher separation rate.RiboPrinter traceability analysis showed that the 7 strains of Pantoea calida had extremely high homology,with a similarity of 0.94±0.03.Conclusion Based on the RiboPrinter traceability and BioNumerics analysis,the contamination of the the powder of traditional Chinese medicine tablets may come from the boiling dryer bag.Corresponding measures should be taken to solve the problem.
作者
王玢
黄演君
潘子康
王乾蕾
WANG Fen;HUANG Yan-jun;PAN Zi-kang;WANG Qian-lei(Zhongshan Institute for Food and Drug Control,Zhongshan Guangdong 528400)
出处
《中南药学》
CAS
2024年第5期1345-1349,共5页
Central South Pharmacy
关键词
微生物污染
泛菌属
溯源分析
microbial pollution
Pantoea calida spp.
traceability analysis
