摘要
目的:探究退黄散对皮肤的刺激作用及过敏反应,为其在临床上的应用提供毒理学依据。方法:家兔皮肤刺激性实验:选取家兔4只,雌雄各半。采用自身对照法,对家兔脊柱两侧进行备皮(3 cm×3 cm),左侧予退黄散,右侧予温水,1次/d,连续给药14 d,观察抹药局部是否有红斑、水肿等现象,对皮肤进行刺激反应及强度评分,末次给药后取给药部位皮肤进行HE染色观察组织病理情况;豚鼠皮肤过敏性试验:选取豚鼠30只,雌雄各半,将其分为空白对照组(温水)、阳性对照组(2,4二硝基氯苯:致敏浓度1%、激发浓度1%)和给药组(退黄散药液适量),每组10只。于第1、7、14天在豚鼠左侧备皮区域致敏,末次致敏结束后第14天对右侧备皮区域进行激发,在激发后1、24、48和72 h观察豚鼠皮肤是否出现红斑、水肿等过敏反应,并计算各组豚鼠反应平均值和致敏率。结果:退黄散对家兔皮肤无刺激,病理检查未见组织病理学改变;在豚鼠皮肤过敏性实验中,皮肤未出现红斑及水肿现象,致敏率为0%。结论:退黄散对家兔皮肤无刺激性,对豚鼠皮肤无过敏性,具备较好的安全性,可在临床推广使用。
Objective:To study the skin irritation and allergic reactions of Tuihuangsan and to provide a toxicological basis for its use in clinical practice.Methods:Skin stimulation experiment in rabbits:4 rabbits,male and female,were used.The skin of both sides of the spine of rabbits was prepared(3 cm×3 cm)by the self-control method,and the left side was administered with Tuihuangsan and the right side was administered with warm water once a day,and the drug was repeatedly administered for 14 days,and the skin was observed for erythema and edema,and the skin was scored for irritation and intensity.Thirty guinea pigs,half male and half female,were selected and divided into a blank control group(warm water),a positive control group(2,4-dinitrochlorobenzene:sensitising concentration of 1%,excitation concentration of 0.1%)and Tuihuangsan group,with 10 guinea pigs in each group.The guinea pigs were sensitized on the left side of the skin preparation area on days 1,7 and 14,and the right side of the skin preparation area was stimulated on day 14 after the last sensitization,and the guinea pigs were observed for erythema,edema and other allergic reactions at 1 h,24 h,48 h and 72 h after the stimulation,and the mean values of reactions and sensitization rates were calculated for each group.Results:The skin of rabbits was not irritated by the antireflux powder,and no histopathological changes were observed in the pathological examination;in the guinea pig skin sensitization test,no erythema and edema were observed in the skin,and the sensitization rate was 0%.Conclusion:Tuihuangsan is non-irritating to the skin of rabbits and non-allergenic to the skin of guinea pigs,which is of good safety and can be promoted for use in the clinic.
作者
蔡敏芳
邓万娟
罗萍
罗玲艳
杨康杰
朱丽
钱海兵
CAI Minfang;DENG Wanjuan;LUO Ping;LUO Lingyan;YANG Kangjie;ZHU Li;QIAN Haibing(Department of Chinese Medicine Pharmacology,School of Basic Medical Sciences,Guizhou University of Traditional Chinese Medicine,Guizhou 550000)
出处
《南通大学学报（医学版）》
2024年第2期128-131,共4页
Journal of Nantong University(Medical sciences)
基金
贵州省普通高等学校特色重点实验室(黔教合KY字[2017]006)。
关键词
退黄散
刺激性
过敏性
家兔
豚鼠
Tuihungsan
irritation
allergic
rabbit
guinea pig
