摘要
目的 观察通督安神方对抑郁模型大鼠前额叶p-JNK、p-p38蛋白表达及血清肿瘤坏死因子-α(TNF-α)含量的影响,并探讨通督安神方抗抑郁机制。方法 将50只大鼠随机分为5组,分别为空白组、模型组、安神组、通督组、通督安神组,每组10只。采用21 d慢性不可预知温和应激(CUMS)造抑郁大鼠模型,造模成功后评估大鼠行为学变化。采用Western Blot法检测p-JNK、p-p38蛋白含量;ELISA法检测大鼠血清TNF-α含量;HE染色法观察大鼠前额叶细胞病理形态。结果 与空白组比较,模型组旷场总距离降低、旷场静止时间增加(P<0.05或P<0.01),糖水偏好率显著下降(P<0.01),前额叶p38、JNK蛋白磷酸化表达水平显著增强(P<0.01),血清TNF-α显著升高(P<0.01),神经元凋亡数量增多。与模型组比较,各治疗组旷场总距离增加、静止不动时间缩短(P<0.05),糖水偏好率显著上升(P<0.01);安神组和通督安神组p-p38、pJNK均降低(P<0.05),血清TNF-α含量均显著下降(P<0.01)、前额叶神经元细胞数量增多。结论 通督安神方可显著改善CUMS大鼠的行为学,降低血清TNF-α含量水平,有效调控JNK/p38 MAPK通路的活化,降低大鼠前额叶p38、JNK磷酸化表达水平,同时修复前额叶受损细胞,发挥抗抑郁作用。
Objective To observe the effects of Tongdu Anshen Prescription on p-JNK,p-p38 protein expression in prefrontal lobe and serum TNF-αcontent in depression model rats,and to explore the anti-depressant mechanism of Tongdu Anshen Prescription.Methods Fifty rats were randomly divided into 5 groups,namely blank group,model group,Anshen group,Tongdu group and Tongdu Anshen group,10 rats each.A 21-day CUMS modelling approach was used to prepare a depressed rat model.After the modelling was successful,the rats were administered drugs and assessed again for behavioural changes.At the end of assessment,Westen Blot was used to detect p-JNK and p-p38 protein contents;ELISA was used to detect serum tumor necrosis factor-α(TNF-α)content;HE staining was used to observe the pathological morphology of prefrontal cells.Results Compared with blank group,model group showed decreased total distance,increased resting time in the open field(P<0.05 or P<0.01),significantly decreased sugar-water preference rate(P<0.01),significantly enhanced prefrontal p38 and JNK protein phosphorylation expression levels(P<0.01),significantly increased serum TNF-α(P<0.01),and increased number of neuronal apoptosis.Compared with model group,the total distance of the open field was increased,the resting time was shortened(P<0.05),and the sugar-water preference rate was significantly increased(P<0.01)in each treatment group;P-p38 and p-JNK were decreased(P<0.05),serum TNF-αlevels were significantly decreased(P<0.01),and the number of prefrontal neuronal cells was increased in both Anshen and Tongdu Anshen groups.Conclusion Tongdu Anshen Prescription can significantly improve the behavior of CUMS rats,reduce the level of serum TNF-α,effectively regulate the activation of JNK/p38 MAPK pathway,reduce the expression level of p38 and JNK phosphorylation in the prefrontal lobe of rats,while repairing damaged cells in the prefrontal lobe and exerting antidepressant effect.
作者
靳宗琳
胡志鹏
章程鹏
JIN Zong-lin;HU Zhi-peng;ZHANG Cheng-peng(Hubei University of Chinese Medicine,Wuhan 430065)
出处
《湖北中医药大学学报》
2024年第2期5-9,共5页
Journal of Hubei University of Chinese Medicine
基金
国家自然科学基金项目(项目编号:81503629)。
关键词
通督安神方
慢性温和不可预知应激
抑郁症
信号通路
凋亡
Tongdu Anshen Prescription
Chronic mild unpredictable stress
Depression
Signal path
Apoptosis
