摘要
目的研究枳壳中3,5,6,7,8,3′,4′-七甲氧基黄酮(HMF)对湿阻中焦大鼠的改善作用。方法将大鼠随机分为正常组、模型组、阳性对照组(消旋山莨菪碱片,1 mg/kg)和HMF低、中、高剂量组(0.3、0.6、0.9 mg/kg),每组7只。除正常组外,其余各组大鼠均采用内因加外因复合因素法造模。造模成功后,各组大鼠灌胃相应药物或生理盐水,每日1次,连续14 d。观察各组大鼠饮食量、饮水量及精神状态等一般行为学状态,测定大鼠粪便含水率及唾液流率。采用苏木素-伊红染色法观察大鼠胃和小肠组织的病理形态学变化;检测大鼠血浆中醛固酮含量;检测大鼠胃组织中水通道蛋白3(AQP3)表达水平。结果与正常组比较,模型组大鼠饮食量、饮水量均显著减少(P<0.01),粪便含水率、唾液流率、血浆中醛固酮含量和胃组织中AQP3表达水平均显著增加/升高(P<0.01);大鼠胃组织损伤侵及黏膜肌层,黏膜肌层断裂;小肠出现少量肠绒毛糜烂,腺体结构破坏等病理形态学变化。与模型组比较,HMF各剂量组大鼠饮食量、饮水量均增加,粪便含水率、唾液流率、血浆中醛固酮含量和胃组织中AQP3表达水平均减少/降低,上述差异大部分有统计学意义(P<0.05或P<0.01);胃和小肠组织的病理形态学变化均有不同程度改善。结论枳壳燥性成分HMF对湿阻中焦大鼠症状有一定的改善作用,其机制可能与降低血浆醛固酮含量、下调胃组织中AQP3表达有关。
OBJECTIVE To investigate the improvement effects of 3,5,6,7,8,3′,4′-heptamethoxyflavone(HMF)of Fructus Aurantii on rats with damp blockage of the middle energizer.METHODS The rats were randomly divided into normal group,model group,positive control group(Raceanisodamine tablet,1 mg/kg),HMF low-dose,medium-dose and high-dose groups(0.3,0.6,0.9 mg/kg),with 7 rats in each group.Except for the normal group,the other groups were modeled by internal and external composite factors.After successful modeling,the rats in each group were given the corresponding drug or normal saline,once a day,for 14 days.The general behavioral states such as dietary intake,water intake and mental state of the rats were observed,and the fecal water content rate and saliva flow rate were measured.Hematoxylin-eosin(HE)staining was used to observe the pathological and morphology in gastric and small intestinal tissues of rats.The plasma content of aldosterone was detected,and the expression of aquaporins(AQP3)in the gastric tissue of rats was determined.RESULTS Compared with the normal group,the dietary intake and water intake of the model group rats were significantly decreased(P<0.01),the fecal water content rate,salivary flow rate,plasma content of aldosterone and the expression of AQP3 in gastric tissue were increased significantly(P<0.01).Gastric tissue injury invaded the mucosal muscle layer,resulting in mucosal muscle layer rupture;pathological and morphological changes such as small intestinal villous erosion and glandular structure destruction were observed in the small intestine.Compared with the model group,the dietary intake and water intake of rats were increased in HMF groups;fecal water content rate,salivary flow rate,plasma content of aldosterone,the expression of AQP3 in gastric tissue were decreased,most of the above differences were statistically significant(P<0.05 or P<0.01).The pathological and morphological changes in the gastric and small intestine tissues of rats had been improved to varying degrees.CONCLUSIONS HMF
作者
龚文慧
谢亚婷
辛力
严世豪
赵蓓蓓
郑郁清
郭静英
商杰
郑鹏
张金莲
GONG Wenhui;XIE Yating;XIN Li;YAN Shihao;ZHAO Beibei;ZHENG Yuqing;GUO Jingying;SHANG Jie;ZHENG Peng;ZHANG Jinlian(School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China)
出处
《中国药房》
CAS
北大核心
2024年第7期819-824,共6页
China Pharmacy
基金
国家自然科学基金项目(No.82060724)
大学生创新创业训练计划项目(No.202310412027)
江西省中医药标委会2021年第二批标准化项目(No.2021B01)。
