摘要
目的了解中缅边境地区中华按蚊成蚊对杀虫剂的抗药性现状,为中国消除疟疾后该区域的媒介控制提供科学依据。方法2023年7月4-26日,在中缅边境地区选取腾冲市、沧源佤族自治县(沧源县)和盈江县作为监测县,在每个县选择1个适宜中华按蚊孳生的自然村作为调查点。每个调查点采集不少于800只雌性吸血中华按蚊带回实验室,次日采用世界卫生组织(WHO)推荐的成蚊接触筒诊断剂量法测定蚊虫对9种杀虫剂的抗性。设立空白对照组,每种剂量杀虫剂重复测定3次取均值,记录杀虫剂接触时间、首只蚊虫击倒时间、60 min蚊虫击倒数以及24 h蚊虫死亡数,计算首只蚊虫击倒时间均值、60 min击倒率、半数击倒时间(KT50)和24 h校正死亡率,χ2检验分别比较3个调查点试虫对每种杀虫剂的24 h校正死亡率差异。杀虫剂标准品和WHO推荐的成蚊接触筒均由中国疾病预防控制中心传染病预防控制所媒介生物控制室提供。结果3个监测县共完成2224只中华按蚊的测定,共测定0.05%溴氰菊酯、0.15%高效氯氟氰菊酯、0.15%高效氟氯氰菊酯、5%马拉硫磷、0.5%氯菊酯、1%杀螟硫磷、0.1%虫威、0.1%残杀威和0.06%氟虫腈9种杀虫剂。结果显示,沧源县调查点中华按蚊成蚊的24 h平均校正死亡率分别为25.68%、26.58%、46.67%、100%、39.19%、86.75%、55.29%、35.96%和25.40%;腾冲市调查点中华按蚊成蚊的24 h平均校正死亡率分别为40.40%、44.58%、34.74%、96.05%、5.00%、97.00%、44.19%、27.96%和63.41%;盈江县调查点中华按蚊成蚊的24 h平均校正死亡率分别为39.73%、49.41%、53.95%、100%、26.47%、100%、91.67%、100%和43.37%。3个调查点试虫对0.15%高效氯氟氰菊酯、0.15%高效氟氯氰菊酯、0.5%氯菊酯、1%杀螟硫磷、0.1%虫威、0.1%残杀威和0.06%氟虫腈7种杀虫剂的24 h平均校正死亡率之间差异均有统计学意义(均P<0.05)。结论中缅边境调查地区中华按蚊对�
Objective To investigate the current status of insecticide resistance of Anopheles sinensis in China-Myanmar border areas,so as to provide scientific basis for vector control in this region after malaria elimination in China.Methods From July 4 to 26,2023,in Tengchong City,Cangyuan Wa Autonomous County(Cangyuan),and Yingjiang County along the China-Myanmar border,one natural villages each suitable for An.sinensis breeding were selected as investigation points.At each survey point,no less than 800 female blood-sucking An.sinensis mosquitoes were collected and brought back to the laboratory,and on the following day,mosquito resistance to nine insecticides was measured using the WHO tube test.Blank control were set up,and three replicates were conducted for each dose of insecticides.The contact time of the insecticide,the first mosquito strike time,the 60 min mosquito strike count,and the number of mosquito deaths at 24 h were recorded.The mean first knockdown time,knockdown rate at 60 min,median knockdown time(KT50),and 24 h corrected mortality were caculated.The Chi-square test was used to compare the 24 h corrected mortality of test insects for each insecticide at the three survey sites.The reference insecticides and the WHOrecommended tubes were both provided by the department of vector biology and control,national institute for communicable disease control and prevention,Chinese center for disease control and prevention.Results The resistance of a total of 2224 An.sinensis mosquitoes were measured at three surveillance counties to nine insecticides including 0.05 %deltamethrin,0.15% lambda-cyhalothrin,0.15% beta-cyfluthrin,5% malathion,0.5% permethrin,1% fenitrothion,0.1% bendiocarb,0.1% propoxur,and 0.06% fipronil.At Cangyuan survey point,the 24 h average corrected mortality rates of An.sinensis adults were 25.68%,26.58%,46.67%,100%,39.19%,86.75%,55.29%,35.96%,and 25.40% for the nine insecticides,respectively;at Tengchong survey point,the 24 h average corrected mortality rates were 40.40%,44.58%,34.74%,96.05%,
作者
曹自有
田鹏
戴雨琪
尹建海
夏志贵
林祖锐
CAO Zi-you;TIAN Peng;DAI Yu-qi;YIN Jian-hai;XIA Zhi-gui;LIN Zu-rui(Department of Malaria Control,National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Chinese Center for Tropical Diseases Research,WHO Collaborating Centre for Tropical Diseases,National Center for International Research on Tropical Diseases,Minisitry of Science and Technology,NHC Key Laboratory of Parasite and Vector Biology,Shanghai 200025,China;Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research,Yunnan International Laboratory for Tropical Infectious Diseases,Yunnan Innovative Team of Key Techniques for Vector Borne Disease Control and Prevention,Yunnan Institute of Parasitic Diseases,Pu’er,Yunnan 665000,China)
出处
《中国媒介生物学及控制杂志》
CAS
2024年第2期244-248,共5页
Chinese Journal of Vector Biology and Control
关键词
中华按蚊
杀虫剂
抗药性
中缅边境
Anopheles sinensis
Insecticides
Resistance
China-Myanmar border
