摘要
探讨我国疟疾消除后,输入性疟疾防控策略的应用及保有实验室检测能力的重要性。在接诊国外输入疫情实战中,评价经典的显微镜检与荧光定量PCR检测疟原虫感染效能。2022年7月28日几内亚务工归国人员在北京新冠肺炎隔离点集中隔离,8月1日检出首例病例,收集同航班人员抗凝血样,采用流行病学研究方法的筛查一致性检验,对所有人员同时进行显微镜检和荧光定量PCR检测。结果显示,126名归国人员中,17例显微镜检发现疟原虫,感染率13.5%。10例荧光定量PCR阳性,感染率7.9%,χ^(2)=2.03,P>0.05,两种检测方法无统计学差异。粗一致率为94.4%,Kappa值0.70,属于中度一致。原虫密度低的感染者荧光定量PCR检测容易漏检。结果表明,我国疟疾消除后,带虫回国人员具有再传播风险。保持显微镜检能力可以做到早发现、早治疗,是控制疟疾输入再传播的有效手段。
To explore the application of prevention and control strategies for imported malaria and the importance of retaining laboratory testing capacity after the elimination of malaria has been achieved in China;to evaluate the efficacy of classical microscopic examination and fluorescence quantitative PCR(qPCR)for detecting Plasmodium infection in screening imported cases from abroad.After the first suspected case of malaria was confirmed on August 1,2022 among the workers returned from Guinea who were being quarantined in COVID-19 Isolation Center in Beijing,anticoagulated blood samples were collected from the travelers in the same flight,and microscopic examination and qPCR tests were performed simultaneously on all personnel using the consistency check of epidemiological methods.Among the 126 returnees,microscopic examination detected 17 cases,with an infection rate of 13.5%;qPCR tests diagnosed 10 cases,with an infection rate of 7.9%.There was no statistical difference between the two methods(χ^(2)=2.03,P>0.05).The rough consistency rate is 94.4%with the Kappa value of 0.70,suggesting a moderate consistency between two methods.Patients with low protozoan density were easy to miss detection by the qPCR test.After the elimination of malaria in China,there are still risks of retransmission by the imported cases from malaria endemic areas.Maintaining the capacity of microscopic examination of malaria enables early detection and treatment,which is an effective method for controlling retransmission by imported cases.
作者
王小梅
吴钶
何战英
吴文婷
杜丹
李旭
WANG Xiao-mei;WU Ke;HE Zhan-ying;WU Wen-ting;DU Dan;LI Xu(Beijing Center for Disease Prevention and Control,Beijing 100013,China;Beijing Chaoyang District Center for Disease Prevention and Control,Beijing 100022,China)
出处
《寄生虫与医学昆虫学报》
CAS
2023年第2期69-73,共5页
Acta Parasitologica et Medica Entomologica Sinica
关键词
疟疾
筛检
原虫密度
显微镜检
荧光定量PCR
Malaria
Screening
Protozoan density
Microscopic examination
Fluorescence quantitative PCR
