摘要
从柴油污染土壤中分离筛选出2株高效石油降解菌株CY-1和CY-2,经形态学观察、生理生化实验及16S rRNA基因序列分析,鉴定CY-1为多舌假单胞菌(Pseudomonas plecoglossicida),CY-2为土地戈登菌(Gordonia terrae)。进一步研究了2株高效菌降解柴油的最佳条件及其对烷烃各组分的降解性能。结果表明:培养温度30℃,pH值为7.0,柴油浓度500 mg/L,接种量8%,NaCl浓度0.5%时,CY-1菌株培养7 d后对柴油的降解率达到65.51%,对链长C15~C25烷烃的平均降解率达到65.36%;培养温度30℃,初始pH值为7.0、柴油浓度3000 mg/L,接种量2%,NaCl浓度0.5%时,CY-2菌株培养7 d后对柴油的降解率达到64.87%,对链长为C15~C25的烷烃的平均降解率达到85.83%。两株菌在柴油污染治理方面均具有一定的应用前景,两株菌相比,CY-2菌株降解柴油的性能优于CY-1菌株。
Two highly efficient petroleum-degrading strains,CY-1 and CY-2,were isolated and screened from soil contaminated with diesel fuel.Following morphological observation,physiological and biochemical experiments,as well as 16S rRNA gene sequence analysis,CY-1 was identified as Pseudomonas plecoglossicida while CY-2 was identified as Gordonia terrae.The optimal conditions for the degradation of diesel oil by these two high-efficiency bacteria strains and their metabolism of straight-chain alkanes were investigated.Experimental results demonstrated that at a culture temperature of 30℃,pH of 7.0,diesel oil concentration of 500 mg/L,inoculation amount of 8%,and NaCl concentration of 0.5%,the degradation rate achieved by CY-1 reached 65.51%after a seven-day culture period with an average degradation rate for C15-C27 alkanes reaching 64.30%.Similarly,when cultured at a temperature of 30°C with an initial pH value of 7.0,a diesel oil concentration of 3000 mg/L,an inoculation amount of 2%,and a NaCl concentration also at 0.5%,the diesel oil degradation rate exhibited by strain CY-2 reached up to approximately 64.87%after seven days with an average degradation rate for alkanes ranging from C14 to C27 reaching approximately 87.73%.Both strains have a certain application prospect in diesel pollution control,and the diesel oil degradation performance of CY-2 strain was better than that of CY-1 strain.
作者
陈应星
王建刚
陈长灏
周郭
徐婷婷
Chen Yingxing;Wang Jiangang;Chen Changhao;Zhou Guo;Xu Tingting(College of Environmental Science and Engineering,West China Normal University,Nanchong 637000,Sichuan,China)
出处
《绿色科技》
2024年第6期191-197,共7页
Journal of Green Science and Technology
基金
西华师范大学博士启动项目(编号:19E058)。
关键词
柴油
菌种鉴定
生物降解
降解特性
diesel oil
strain identification
biodegradation
degradation characteristics
