摘要
为了探讨松鼠葡萄球菌(Staphylococcus sciuri)的病原学特性,本试验从耗儿鱼(Corydoras)分离出菌株YDE0916,并对其进行生理生化鉴定、16SrDNA基因PCR扩增、序列分析及耐药性分析。结果显示,该菌株为革兰氏阳性球菌,呈串形,无芽孢,无荚膜。该菌不运动,β-半乳糖苷酶、VP反应为阴性,蔗糖、麦芽糖为阳性。扩增的16SrDNA基因序列长度为1 476bp(GenBank登录号:KP031644),与GenBank中的松鼠葡萄球菌16SrDNA基因序列的相似性达99%。系统进化树显示,菌株YDE0916和松鼠葡萄球菌聚为一簇,由此判定菌株YDE0916为松鼠葡萄球菌。药敏试验结果显示,该菌株对诺氟沙星、杆菌肽耐药,对美满霉素、庆大霉素、氨苄西林等22种药物高度敏感。本研究为松鼠葡萄球菌的分离鉴定及预防积累参考依据。
To explore etiology characteristics of Staphylococcus sciuri,strain YDE0916 was isola- ted and identified from Corydoras by cultivation, biochemical identification, 16S rDNA gene PCR amplification and sequence analysis, and resistance analysis. The result showed this strain was gram-positive, serial, spore, without capsule. The physicochemical properties showed immobility, β-galactosidase-negative, VP reaction-negative, sugar and maltose-positive. The nucleotide sequence of 16S rDNA was 1 476 bp and its GenBank accession number was KP031644. This 16S rDNA showed 99% homology with Staphylococcus sciuri. Phylogenetic tree showed that strain YDE0916 and several Staphylococcus sciuri strains gathered into one cluster. So the strain was identified as Staphylococcus sciuri. The antibiotic sensitive test showed that strain YDE0916 was resistant to norfloxacin and bacitracin, and was highly sensitive to 22 kinds of drugs, such as minocycline,gentamicin and ampicillin. This study could provide a reference for identification and prevention of Staphylococcus sciuri.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第5期1288-1293,共6页
China Animal Husbandry & Veterinary Medicine
基金
四川省科技支撑项目(2013FZ0014)
西南民族大学研究生创新型科研项目(CX2015SZ098)
西南民族大学研究生学位点建设项目(2014XWD-SO71007)
