摘要
白辣椒是浙江省最具区域特色的辣椒类型,了解其遗传多样性是推动白辣椒新品种选育的重要依据。该研究利用Hyper-seq简化基因组测序对国内外收集的64份白辣椒高代自交系进行简化基因组测序,并进行主成分分析、聚类分析及群体结构分析。研究结果发现,在辣椒12条染色体上,共获得3511122个单核苷酸多态性(single nucleotide polymorphism,SNP)位点和922513个插入缺失标记(insertion-deletion,InDel),平均每条染色体的SNP和InDel变异数量分别为292593.5和76876.0。注释结果表明,SNP和InDel主要发生在基因间隔区,数量多达4000310,其次为内含子区域,数量为226301。基于SNPs多样性的聚类分析和群体结构分析均揭示64份地方白辣椒材料可分为2个类群,一个类群包含36份鲜食型白辣椒和1份观赏型白辣椒,另一个类群包含27份观赏型白辣椒。主成分分析中,两个类群分别聚类于PC1和PC2两个不同区域。研究结果揭示了白辣椒种质资源的群体结构和遗传多样性,为今后的白辣椒种质开发和利用奠定基础。
White-fruited pepper is the most regionally characteristic type of pepper(capsicum)in Zhejiang Province,understanding its genetic diversity serves as an essential foundation for promoting the breeding of new varieties of white-fruited pepper.In this study,64 white-fruited pepper inbred lines collected from China and abroad were sequenced by Hyper-seq.Principal component analysis,cluster analysis and population structure analysis were also performed.The results showed that a total of 3511122 single nucleotide polymorphism(SNP)variation and 922513 insertion-deletion(InDel)were identified from 12 chromosomes of pepper.The average number of SNPs and InDels per chromosome was 292593.5 and 76876.0,respectively.The annotation results revealed that InDel and SNP variation predominantly manifested in the intergenic regions,reaching a quantity of 4000310.The next significant occurrence was within intronic regions,totaling 226301.Cluster analysis and population structure analysis,based on SNP diversity,classified the 64 white pepper materials into two main groups:one comprised 36 fresh white-fruited pepper and 1 ornamental white-fruited pepper,while the other consisted of 27 ornamental white-fruited pepper.The principal component analysis results indicated that two groups clustered distinctly in the PC1 and PC2 regions.This study unveils the genetic structure and genetic diversity of white-fruited pepper germplasm resources,providing a foundational understanding for the future development and application of white-fruited pepper germplasm.
作者
方萍萍
张婷
韦静
王雪艳
李朝森
刘慧琴
万红建
郭勤卫
FANG Pingping;ZHANG Ting;WEI Jing;WANG Xueyan;LI Chaosen;LIU Huiqin;WAN Hongjian;GUO Qinwei(Quzhou City Academy of Agricultural and Forestry Sciences,Quzhou 324000,Zhejiang;Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang)
出处
《浙江农业科学》
2024年第5期1056-1063,共8页
Journal of Zhejiang Agricultural Sciences
基金
浙江省蔬菜育种专项项目(2021C02065-1-4)
衢州市科技局项目(2021K15,2022K22)。
