摘要
The neuropathological features of Alzheimer’s disease include amyloid plaques.Rapidly emerging evidence suggests that Piezo1,a mechanosensitive cation channel,plays a critical role in transforming ultrasound-related mechanical stimuli through its trimeric propeller-like structure,but the importance of Piezo1-mediated mechanotransduction in brain functions is less appreciated.However,apart from mechanical stimulation,Piezo1 channels are strongly modulated by voltage.We assume that Piezo1 may play a role in converting mechanical and electrical signals,which could induce the phagocytosis and degradation of Aβ,and the combined effect of mechanical and electrical stimulation is superior to single mechanical stimulation.Hence,we design a transcranial magneto-acoustic stimulation(TMAS)system,based on transcranial ultrasound stimulation(TUS)within a magnetic field that combines a magnetoacoustic coupling effect electric field and the mechanical force of ultrasound,and applied it to test the above hypothesis in 5xFAD mice.Behavioral tests,in vivo electrophysiological recordings,Golgi–Cox staining,enzyme-linked immunosorbent assay,immunofluorescence,immunohistochemistry,real-time quantitative PCR,Western blotting,RNA sequencing,and cerebral blood flow monitoring were used to assess whether TMAS can alleviate the symptoms of AD mouse model by activating Piezo1.
出处
《Research》
SCIE
EI
CSCD
2024年第1期81-95,共15页
研究（英文）
基金
the National Natural Science Foundation of China(81927806,52107241,and 52077223)
Chinese Academy of Medical Sciences Medical and Health Science and Technology Innovation Project Mission Statement(2021-I2M-1-058)
CAMS Innovation Fund for Medical Sciences(2022-I2M-2-003).
