摘要
背景:机械敏感通道蛋白Piezo1是机械应力刺激的重要靶蛋白,能将物理的机械力转化成生物电信号,从而调控骨形成与骨吸收,在抗骨质疏松中发挥着重要作用。目的:总结机械应力刺激在抗骨质疏松中的作用机制、Piezo1分子生物学研究进程及Piezo1在骨质疏松中的研究进展。方法:使用计算机在中国知网、万方、维普、PubMed及Web of Science数据库进行文献检索,中文检索词为“骨质疏松、Piezo1、机械应力、骨”,英文检索词为“osteoporosis,Piezo1,mechanical,osteoblast,osteoclast,chondrocyte,bone”,根据纳排标准对文献进行筛选,最终纳入65篇文献进行综述。结果与结论:①机械敏感通道蛋白Piezo1的蛋白结构在冷冻电镜技术突破中不断得到新的解析,目前研究表明Piezo1蛋白基于三叶螺旋桨状三维构造主体,在静默和应激状态下可表现出不同的结构变化;②Piezo1介导的机械应力刺激能通过调控成骨细胞、软骨细胞、内皮细胞、肠道细胞的功能影响骨形成,同时调控破骨细胞的功能影响骨吸收,进而在抗骨质疏松中发挥重要作用;③Piezo1介导的机械应力刺激主要通过Wnt/β-catenin信号通路调控成骨细胞功能影响骨形成;而其对破骨细胞功能的调节,因机械力的类型、大小、作用时间的不同而对骨吸收有双向调节作用,需进一步量化的研究;④未来基于Piezo1在骨质疏松中的临床研究、骨质疏松动物模型研究、破骨细胞的相关机制及新型复合材料研究仍需更多的关注。
BACKGROUND:Piezol is a mechanosensitive channel that acts as an important target protein for mechanical stress stimulation.Piezol conve rts external mechanical forces into biological signals,regulating bone formation and bone resorption.Piezol plays an important role in anti-osteoporosis.OBJECTIVE:To summarize the mechanism of mechanical stress stimulation in anti-osteoporosis and research progress in Piezol molecular biology as well as the use of Piezol in the treatment of osteoporosis.METHODS:Relevant literature was retrieved in CNKI,Wan Fang,VIP,PubMed,and Web of Science databases using computers.The search terms were"osteoporosis,Piezol,mechanical,osteoblast,osteoclast,chondrocyte,bone in Chinese and English.Finally,65 documents were included for review according to the inc usion and exclusion criteria.RESULTS AND CONCLUSION:The structure of Piezol has been continuously analyzed in the breakthrough of cryo-electron microscopy.Based on a three-bladed propeller 3D structure,Piezol exhibits different structural changes under silent and stressful states.Piezol-mediated mechanical stress stimulation can affect bone formation by regulating the functions of osteoblasts,chondrocytes,endothelial cells and intestinal cells,and simultaneously affect bone resorption by regulating the function of osteoclasts,thereby playing an important role in anti-osteoporosis.Piezol-mediated mechanical stress mainly affects bone formation by regulating the function of osteoblasts through the Wnt/β-catenin signaling pathway.And it has a two-way regulatory effect on osteoclasts due to different type,strength,and duration of mechanical fo rce,which needs to be further quantified.For the use of Piezol in the treatment of osteoporosis,emphasis should be placed on clinical and animal model research for osteoporosis,osteoclast-related mechanisms,and new composite materials.
作者
黄浩然
卫杨文祥
章家皓
莫亮
刘予豪
陈镇秋
王海彬
周驰
Huang Haoran;Wei Yangwenxiang;Zhang Jiahao;Mo Liang;Liu Yuhao;Chen Zhenqiu;Wang Haibin;Zhou Chi(First School of Clinical Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第17期2716-2722,共7页
Chinese Journal of Tissue Engineering Research
基金
2021年国家自然科学基金项目(82074462),项目负责人:王海彬
2021年国家自然科学基金项目(82104883),项目负责人:刘予豪
2021年度广东省普通高校特色创新类项目(2021KTSCX021),项目负责人:周驰
2021年广州市科技计划项目(202102020930),项目负责人:刘予豪
广州中医药大学“双一流”与高水平大学学科协同创新团队项目(2021xk46),项目负责人:陈镇秋。
