摘要
目的探讨黄连素治疗大鼠种植体周围炎的作用以及对Toll样受体4(Toll-like receptor 4,TLR4)/核因子κB(nuclear factorκB,NF-κB)信号通路的影响。方法建立SD大鼠种植体周围炎大鼠模型,随机分为模型组、黄连素低剂量组、黄连素高剂量组和抗生素组,另设阴性对照组。黄连素低、高剂量组予以100、200 mg·kg^(-1)黄连素,抗生素组予以200 mg·kg^(-1)阿莫西林胶囊和100 mg·kg^(-1)甲硝唑片,模型组和阴性对照组予以等体积生理盐水,连续干预4周。观察各组大鼠种植体一般情况,用牙周探针测量大鼠种植体探诊深度(probing depth,PD)和牙龈指数(gingival index,GI);计算机断层扫描(computed tomography,CT)测量大鼠种植体周围骨高度与骨密度;用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测大鼠种植体周围牙龈组织中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白细胞介素-6(interleukin-6,IL-6)的含量;蛋白印迹法(Western blotting)检测大鼠种植体周围牙龈组织中TLR4/NF-κB信号通路相关蛋白的表达情况。结果模型组、黄连素低剂量组、黄连素高剂量组、抗生素组及阴性对照组大鼠PD和GI指数逐渐降低,种植体周围骨高度、骨密度逐渐升高,牙龈组织中TNF-α、IL-1β水平逐渐降低,种植体龈沟液中Ⅱ型胶原(collagen-Ⅱ,COL-Ⅱ)和基质金属蛋白酶13(matrix metalloproteinase-13,MMP-13)活性逐渐升高(P<0.05)。模型组、黄连素低剂量组和黄连素高剂量组及阴性对照组,大鼠种植体周围牙龈组织中TLR4、MyD88和p-NF-κB-65蛋白的相对表达量逐渐降低(P<0.05)。结论黄连素对大鼠种植体周围炎有一定治疗作用,其机制可能与抑制TLR4/NF-κB信号通路有关。
Objective To investigate the effect of berberine in the treatment of peri-implantitis in rats and its effect on Toll-like receptor 4(TLR4)/nuclear factorκB(NF-κB)signaling pathway.Methods SD rats were used to build the peri-implantitis model,and randomly divided into model group,low-dose and high-dose berberine groups and antibiotic group,and a negative control group was set up.The low-dose and high-dose berberine groups were given 100 and 200 mg·kg^(-1) berberine,the antibiotic group was given 200 mg·kg^(-1) Amoxicillin Capsules and 100 mg·kg^(-1) Metronidazole Tablets,the model group and the negative control group were given equal volume of normal saline,continuous intervention for 4 weeks.The general conditions of the implants in each group were observed,the probing depth(PD)and gingival index(GI)of the implants were measured with a periodontal probe.The bone height and bone mineral density around the implants were measured by computed tomography(CT).The contents of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in peri-implant gingival tissue were detected by enzyme linked immunosorbent assy(ELISA).The TLR4/NF-κB signaling pathway-related protein expression in peri-implant gingival tissue of rats were detected by Western blotting.Results In the model,low-dose berberine,high-dose berberine,antibiotic and negative control groups,the PD and GI indexes of the rats gradually decreased,the bone height and bone density around the implant gradually increased,and the levels of TNF-αand IL-1βin the gingival tissue gradually decreased,and the activities of collagen-Ⅱ(COL-Ⅱ)and matrix metalloproteinase-13(MMP-13)in the gingival crevicular fluid of the implant gradually increased(P<0.05).In the model,the low-dose berberine,high-dose berberine and negative control group,the relative expressions of TLR4,MyD88 and p-NF-κB-65 proteins in the gingival tissue around the implants of the rats gradually decreased(P<0.05).Conclusion Berberine has a certain therapeutic effect on periimplantitis in rats,and its m
作者
许楠
赵雁焕
王雯
XU Nan;ZHAO Yanhuan;WANG Wen(Department of Implantology,Tangshan Union Medical College Hospital,Tangshan 063000,China)
出处
《西北药学杂志》
CAS
2024年第3期59-64,共6页
Northwest Pharmaceutical Journal
基金
2020年度中医药类科研计划课题(编号:2020423)。
