摘要
PCR技术作为一项革命性的分子生物学工具,在基因分析、病原体检测、药物研发、遗传学研究等领域发挥着重要作用。384孔PCR仪作为高通量PCR仪的代表,具备一次性处理多个样本的能力。由于现有的校准规范主要针对96孔,使用384孔时存在校准困难和准确性较差的问题。因此,本文通过对两者进行对比,提出了384孔PCR仪的校准布点方式,并进行试验验证。结果表明:采用该校准布点方式具有有效性。
PCR technology,as a revolutionary molecular biology tool,plays an important role in gene analysis,pathogen detection,drug development,genetic research and other fields.As a representative of high-throughput PCR instrument,the 384 well PCR instrument has the ability to process multiple samples at one time.Because the existing calibration specification is mainly for 96 holes,there are problems of calibration difficulty and poor accuracy when using 384 holes.Therefore,through the comparison of the two,this paper puts forward the calibration layout method of 384-well PCR instrument,and carries out the test verification.The experiment shows that this method is effective.
作者
郭振宇
杨丽婷
吴梦言
姚乾文
GUO Zhenyu;YANG Liting;WU Mengyan;YAO Qianwen
出处
《计量与测试技术》
2024年第4期116-118,共3页
Metrology & Measurement Technique
基金
甘肃省市场监督管理局科技计划项目(项目编号:SSCJG-ZJ-A202206)。
关键词
PCR仪
384孔PCR仪
温度校准
PCR instrument
384-well PCR instrument
temperature calibration
