摘要
为研究鸡腹脂中四种不同天然色素含量的HPLC检测方法,实验对5种不同来源的鸡腹脂进行色素成分的检测分析。样品经含有0.5%BHT的二氯甲烷/乙腈(3:2,V/V)混合溶剂萃取,10%无水硫酸钠溶液及正己烷分离,有机相合并浓缩后正己烷溶解定容,部分溶液加入2 mL的40%氢氧化钾甲醇溶液涡旋皂,1 mL醋酸酸化后利用10%无水硫酸钠溶液及正己烷分离,有机相合并浓缩后正己烷溶解,利用无水硫酸钠分别将皂化前后溶液除水后0.22μm有机滤膜过滤上机检测;液相条件为正己烷/乙酸乙酯/异丙醇(730:270:15,V/V/V)等度洗脱,流速1.4 mL/min,445 nm波长进行检测。结果表明:本研究所建方法测定线性范围为0.31~1.57μg/mL,R^(2)=0.9999,RSD为2.68%~4.81%,检出限为0.05 mg/kg,当叶黄素添加量为0.8~3.3 mg/kg时,回收率为96.74%~109.10%;该方法能够满足同时检测鸡腹脂中四种不同天色黄色素成分含量,灵敏度高,重现性好,为研究鸡腹脂中黄色素的着色规律提供技术支持。
To determine the content of four different natural pigments in chicken belly fat by HPLC,and to analyze the pigment components of five different sources of chicken belly fat.Samples were extracted with 0.5%BHT of methylene chloride/acetonitrile(3:2,V/V)mixed solvent,10%anhydrous sodium sulfate solution and n-hexane separation,organic phase merged and concentrated,n-hexane dissolution and constant volume,part of the solution was added to 2 mL of 40%potassium hydroxide methanol solution vortex soap.After 1ml acetic acid acidification,10%anhydrous sodium sulfate solution and n-hexane were separated.The organic phase was merged and concentrated,and n-hexane was dissolved.The solution before and after saponification was dehydrated by anhydrous sodium sulfate and 0.22μm organic filter membrane was used for machine detection.The liquid phase was equal elution of n-hexane/ethyl acetate/isopropyl alcohol(730:270:15,V/V/V)at the flow rate of 1.4 mL/min and wavelength of 445 nm.The results showed that the linear range of this method was 0.31~1.57μg/mL,R^(2)=0.9999,RSD were 2.68%~4.81%,and the detection limit was 0.05 mg/kg.When the dosage of lutein was 0.8~3.3 mg/kg,the recoveries were 96.74%~109.10%.The method could simultaneously detect the content of four different yellow pigments in chicken belly fat,with high sensitivity and good reproducibility,and provide technical support for the study of the coloring rule of yellow pigment in chicken belly fat.
作者
翟彦伟
聂玲玲
吕润豪
宋伟光
ZHAI Yanwei;NIE Lingling;LYU Runhao;SONG Weiguang(Chenguang Biotech Group Co.Ltd.,Handan,Hebei Province 057250,China;Hebei Province Plant Source Animal Health Products Technology Innovation Center,Handan,Hebei Province 057250,China;Hebei Key Laboratory of Comprehensive Utilization of Plant Resources,Handan,Hebei Province 057250,China)
出处
《中国饲料》
北大核心
2024年第9期107-112,共6页
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