摘要
为探究黄芪多糖(APS)和茯苓多糖(PCP)对长江鲟幼鱼不同组织器官抗氧化能力和免疫能力的影响,试验将90尾长江鲟幼鱼随机分为对照组、黄芪多糖组和茯苓多糖组,每组3个重复,每个重复10尾试验鱼,在基础日粮中按照体质量添加黄芪多糖和茯苓多糖,采用灌服方式投喂,试验周期为20 d。试验结束时每个试验组随机选取15尾鱼采集血清、肝脏、肾脏、肌肉、肠道、体表黏液等组织器官,采用双抗体夹心法检测抗氧化指标和免疫指标的变化情况,并分析植物多糖对长江鲟幼鱼不同组织器官抗氧化和免疫指标的影响程度。结果表明:(1)与对照组相比,黄芪多糖可显著增强长江鲟幼鱼血清、肝脏、肾脏、肠道、肌肉等组织中超氧化物歧化酶(SOD)的活性(P<0.05),提高率分别为43.36%、60.27%、43.75%、64.65%和56.87%;可显著增强血清、肝脏、肠道、体表黏液等组织中过氧化氢酶(CAT)的活性(P<0.05),提高率分别为27.97%、38.83%、126.43%和47.93%;可显著增强血清、肾脏、肌肉等组织中过氧化物酶(POD)的活性(P<0.05),提高率分别为12.39%、22.80%和14.14%;可显著增强血清、肝脏、肾脏、肠道、肌肉、体表黏液等组织中谷胱甘肽过氧化物酶(GSH-Px)的活性(P<0.05),提高率分别为24.34%、33.96%、34.65%、25.00%、15.73%和38.96%;可显著增强血清、肝脏、肾脏、肌肉、体表黏液等组织中总抗氧化能力(T-AOC)(P<0.05),提高率分别为16.88%、20.36%、10.59%、12.07%和13.56%;可显著降低血清、肝脏、肾脏、肠道、肌肉、体表黏液等组织中丙二醛(MDA)的含量(P<0.05),降低率分别为29.14%、25.18%、18.15%、28.53%、22.20%和24.40%。茯苓多糖可显著增强长江鲟幼鱼肠道、肌肉等组织中SOD的活性(P<0.05),提高率分别为71.95%和43.00%;可显著增强肾脏、体表黏液等组织中CAT的活性(P<0.05),提高率分别为23.25%和51.29%;可显著增强血清中POD的活性(P<0.05),提高率为16.
To investigate the effects of Astragalus polysaccharides and Poria cocos polysaccharides on the antioxidant and immune abilities of different tissues and organs in juvenile Acipenser dabryanus.In the experiment,90 young Acipenser dabryanus fish were randomly divided into a control group,APS group,and PCP group.Each group had 3 replicates,with 10 experimental fish per replicate.Astragalus polysaccharides and Poria cocos polysaccharides were added to the basic diet according to their body weight,and were fed by gavage.The experimental period was 20 days.At the end of the experiment,15 experimental fish were randomly selected from each experimental group to collect tissues or organs such as blood,liver,kidneys,muscles,intestines,and body surface mucus.The double antibody sandwich method was used to detect antioxidant and immune indicators,and the impact of plant polysaccharides on the antioxidant and immune indicators of different tissues and organs of juvenile Acipenser dabryanus was analyzed.The results showed that:(1)Compared with the control group,Astragalus polysaccharides significantly enhanced the activity of SOD in tissues such as serum,liver,kidney,intestine,and muscle of juvenile Acipenser dabryanus(P<0.05),with an increase rate of 43.36%,60.27%,43.75%,64.65%,and 56.87%,respectively;It could significantly enhance the activity of CAT in tissues such as serum,liver,intestine,and body surface mucus(P<0.05),with an increase rate of 27.97%,38.83%,126.43%,and 47.93%,respectively;It could significantly enhance the activity of POD in tissues such as serum,kidneys,and muscles(P<0.05),with an increase rate of 12.39%,22.80%,and 14.14%,respectively;It could significantly enhance the activity of GSH-Px in tissues such as serum,liver,kidneys,intestines,muscles,and body surface mucus(P<0.05),with an increase rate of 24.34%,33.96%,34.65%,25.00%,15.73%,and 38.96%,respectively;It could significantly enhance T-AOC in tissues such as serum,liver,kidneys,muscles,and body surface mucus(P<0.05),with an increase rate of 16.88%,20
作者
张建明
张德志
田甜
舒德斌
ZHANG Jianming;ZHANG Dezhi;TIAN Tian;SHU Debin(Institute of Chinese Sturgeon Research,China Three Gorges Project Corporation,Yichang,Hubei Province 443100,China;Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes,Yichang,Hubei Province 443100,China)
出处
《中国饲料》
北大核心
2024年第9期96-106,共11页
China Feed
基金
中国长江三峡集团有限公司科技计划项目(WWKY-2021-0003,JG/18001B)。
关键词
长江鲟幼鱼
黄芪多糖
茯苓多糖
抗氧化指标
免疫指标
Acipenser dabryanus
Astragalus polysaccharides
Poria cocos polysaccharides
antioxidant index
immune index
