摘要
文章旨在探究黄曲霉毒素对肉鸡肝脏代谢化合物的影响。试验以白羽肉鸡为试验对象,通过灌服方式注射黄曲霉毒素。利用超高效液相色谱-四极杆-飞行时间质谱,采用非靶向代谢组学分析方法对肝脏中的代谢物进行定量分析,通过主成分分析(principal component analysis,PCA)、聚类分析等统计方式对代谢物的定量结果进行统计分析。结果表明,不同浓度的黄曲霉毒素对白羽肉鸡肝脏代谢造成不同程度的影响,共寻找242个差异化合物。高浓度的黄曲霉毒素对肝脏的损害程度较大,差异化合物最多,以上差异化合物的变化说明黄曲霉毒素可以扰乱肉鸡正常的生理功能,致使肝脏功能受到损伤,为肉鸡黄曲霉毒素内源性生物标志物的筛选奠定理论基础。
The article aims to explore the effect of aflatoxin on metabolic compounds in the liver of broiler chickens.The experiment takes white feathered broiler chickens as the experimental subjects and injects aflatoxin through oral administration.Using ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry,non targeted metabolomics analysis methods were used to quantitatively analyze metabolites in the liver.Statistical methods such as principal component analysis and cluster analysis were used to statistically analyze the quantitative results of metabolites.The results showed that different concentrations of aflatoxin had varying degrees of impact on liver metabolism in white feathered broilers,and a total of 242 differential compounds were identified.High concentrations of aflatoxin cause significant damage to the liver,with the most differential compounds.The changes in these differential compounds indicate that aflatoxin can disrupt the normal physiological functions of broiler chickens,leading to liver damage.This lays a theoretical foundation for the screening of endogenous biomarkers of aflatoxin in broiler chickens.
作者
张蓉银
杜婷婷
魏佩玲
王晓涛
胡波
ZHANG Rongyin;DU Tingting;WEI Peiling;WANG Xiaotao;HU Bo(Institute of animal husbandry quality standards,Xinjiang Academy of animal husbandry Sciences,Urumqi,Xinjiang Uygur Autonomous Region 830000,China)
出处
《中国饲料》
北大核心
2024年第4期9-12,共4页
China Feed
基金
基于UPLC-QTOF/MS代谢组学技术对肉鸡中黄曲霉毒素代谢规律研究(2020D04018)。
