摘要
目的:探讨牙周炎患者唾液微生物对抑郁大鼠行为和炎症因子的影响。方法:使用慢性不可预知轻度应激法(Chronic unpredictable mild stress,CUMS)构建抑郁大鼠模型。造模结束后,对照组和CUMS组磷酸缓冲盐溶液(Phosphate buffered saline,PBS)PBS灌胃,H-CUMS组灌胃30名健康个体的唾液微生物(healthy salivary microbiota,HSM),P-CUMS组灌胃30名牙周炎患者的唾液微生物(periodontitis salivary microbiota,PSM),每隔一天灌胃1次,共2周。灌胃结束后对所有大鼠进行旷场实验和强迫游泳实验测试,取外周血血清,ELISA方法检测炎症因子IL-6和TNF-a的含量。结果:P-CUMS组的运动距离减少(P=0.022),平均速度减慢(P=0.019),绝望行为增加(P=0.030),外周血中IL-6(P=0.015)和TNF-a(P=0.048)含量明显升高,与H-CUMS组相比均有统计学差异。结论:牙周炎患者的唾液微生物加剧了抑郁大鼠的抑郁样行为和炎症反应,预防牙周病的发生,对MDD的治疗和预后起重要作用。
Objective:To investigate the effects of salivary microbiota in periodontitis patients on behavior and inflammatory factors in depressed rats.Methods:Chronic unpredictable mild stress(CUMS)was used to construct a depressed rat model.After molding,Control group and CUMS group were administrated with PBS.H-CUMS group was administrated with healthy salivary microbiota(HSM)from 30 healthy individuals,and P-CUMS group was administrated with periodontitis salivary microbiota(PSM)from 30 patients with periodontitis,once every other day.After 2 weeks of gavage,all the rats were subjected to open field experiment and forced swimming experiment to test their depression-like behavior.The levels of inflammatory factors IL-6 and TNF-a in rat peripheral blood serum were detected by ELISA.Results:Compared with the Control group,the CUMS group had reduced motor distance(P=0.022),slowed down average speed(P=0.019),increased despair behavior(P=0.030),and increased the contents of IL-6(P=0.015)and TNF-a(P=0.048)in peripheral blood.There were statistical differences in all the above.Conclusion:Saliva microbiota from periodontitis patients exacerbated depression-like behavior and inflammatory responses in depressed rats.The prevention of periodontal disease may be play an important role in the treatment and prognosis of MDD.
作者
李琰
张宇涛
闫倩
Li Yan(Department of Stomatology,Fenyang College of Shanxi Medical University,Luliang Shanxi 032200)
出处
《黑龙江医药》
CAS
2024年第2期254-257,共4页
Heilongjiang Medicine journal
基金
山西省高校科技创新计划项目(编号:2023L477)。
