摘要
目的 观察百事乐胶囊调控SHH/Gli1信号通路对抑郁模型大鼠海马神经再生的影响及作用机制。方法 32只SD大鼠随机分为空白组、模型组、氟西汀组(5.4 mg/kg)和百事乐胶囊组(2.88 g/kg),每组8只。采用慢性不可预见性温和应激加单笼饲养法建立抑郁大鼠模型,于造模同时给药,连续21 d。糖水偏好实验、旷场实验评价大鼠抑郁样行为,ELISA检测大鼠血清和海马组织脑源性神经营养因子(BDNF)含量,免疫荧光染色观察海马齿状回5-溴脱氧尿嘧啶核苷(BrdU)、BrdU/双皮质素(DCX)、BrdU/神经元特异性核蛋白(NeuN)阳性细胞数,免疫荧光、Western blot检测大鼠海马组织SHH、Gli1及跨膜转导蛋白Smo、Ptch荧光强度和蛋白表达。结果 与空白组比较,模型组大鼠糖水偏好度明显降低,水平和垂直运动次数明显减少(P<0.01),血清、海马组织BDNF含量明显下降(P<0.05),海马齿状回BrdU、BrdU/DCX、BrdU/NeuN阳性细胞数明显减少(P<0.01),海马组织SHH、Gli1、Smo、Ptch荧光强度及蛋白表达均明显降低(P<0.01,P<0.05);与模型组比较,氟西汀组和百事乐胶囊组大鼠糖水偏好度明显升高,水平和垂直活动次数显著增加(P<0.05,P<0.01),血清、海马组织BDNF含量明显上升(P<0.05,P<0.01),海马齿状回BrdU、BrdU/DCX、BrdU/NeuN阳性细胞数明显增加(P<0.01,P<0.05),海马组织SHH、Gli1、Smo、Ptch荧光强度和蛋白表达显著升高(P<0.01,P<0.05)。结论 百事乐胶囊可通过调控SHH/Gli1信号通路促进抑郁模型大鼠海马神经再生,进而发挥抗抑郁作用。
Objective To explore the effects and mechanism of Baishile Capsules regulating SHH/Gli1 signaling pathway on hippocampal neurogenesis of depression model rats.Methods Totally 32 SD rats were randomly divided into control group,model group,fluoxetine(5.4 mg/kg)group and Baishile Capsules(2.88 g/kg)group,with 8 rats in each group.A depression rat model was established using chronic unpredictable mild stress and single cage feeding method. The model was established and administered simultaneously for 21 consecutive days. Depression-likebehavior in rats were evaluated by sucrose preference experiment and open field experiment, ELISA was used todetect brain derived neurotrophic factor (BDNF) contents in rat serum and hippocampal tissue, the number of BrdU,BrdU/DCX, BrdU/NeuN positive cells in dentate gyrus of the hippocampus was observed by immunofluorescence,immunofluorescence and Western blot were used to detect the fluorescence intensity and protein expression of SHH,Gli1, Smo, Ptch in hippocampal tissue. Results Compared with the control group, the degree of sucrose preferencesignificantly decreased in the model group (P<0.01), the number of horizontal and vertical movements significantlydecreased (P<0.01), the contents of BDNF in serum and hippocampal tissue significantly decreased (P<0.05), thenumber of BrdU, BrdU/DCX, BrdU/NeuN positive cells in dentate gyrus of the hippocampus significantly decreased(P<0.01), and the fluorescence intensity and protein expression of SHH, Gli1, Smo, Ptch in hippocampal tissuesignificantly decreased (P<0.01, P<0.05). Compared with the model group, the degree of sucrose preference and thenumber of horizontal and vertical movements in fluoxetine group and Baishile Capsule group increased significantly(P<0.05, P<0.01), the contents of BDNF in serum and hippocampal tissue significantly increased (P<0.05, P<0.01),and the number of BrdU, BrdU/DCX, BrdU/NeuN positive cells in dentate gyrus of the hippocampus significantlyincreased (P<0.01, P<0.05), the fluorescence intensity and protein
作者
骆妍
蔡川
侯光菡
蔺晓源
杨蕙
吴梅
孟盼
LUO Yan;CAI Chuan;HOU Guanghan;LIN Xiaoyuan;YANG Hui;WU Mei;MENG Pan(Science and Technology Innovation Center,Hunan University of Chinese Medicine,Changsha 410208,China;Forth Hospital of Changsha,Changsha 410000,China;The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2024年第3期98-104,共7页
Chinese Journal of Information on Traditional Chinese Medicine
基金
湖南省自然科学基金优秀青年基金(2020JJ3027)
湖南省科技创新人才计划优秀博士后创新人才项目(2020RC2060)
湖南省中医药科研课题(B2023021)
湖南省教育厅科学研究项目(18C0372)
长沙市杰出创新青年培养计划(kq2009018)
长沙市自然科学基金(kq2208471)。
