摘要
目的:探讨电针对慢性应激抑郁大鼠行为功能及海马CA3区突触可塑性的影响。方法:将144只雄性SD大鼠按随机数字表法分为空白组、模型组、电针组、西药组,每组36只,每组按干预时间分为7d、14d、21d3个亚组,各亚组12只。除空白组外,余组采用慢性温和不可预见性应激加孤养的方式建立抑郁大鼠模型,且干预治疗均与造模同时进行,电针组采用疏密波刺激"神庭""百会"穴,西药组采用灌胃给药氟西汀,每日1次。通过旷场试验(open-field test)、糖水消耗试验和透射电镜,观察大鼠行为学及海马CA3区神经元突触的变化。结果:在实验第7、14、21d,模型组大鼠open-field test评分、糖水消耗量、体质量均较空白组明显降低(均P<0.01),实验第14、21d,电针组、西药组大鼠open-field test评分、糖水消耗量、体质量均较模型组明显提高(P<0.01,P<0.05);实验第14、21d,模型组大鼠海马CA3区突触数量均较空白组明显减少(均P<0.01),电针组、西药组大鼠海马CA3区突触数量均较模型组明显增加(P<0.01,P<0.05);模型组大鼠海马CA3区神经元细胞从实验第7d开始出现相对固缩、变形,突触结构融合、界限不清,21d时上述改变显著,电针组、西药组大鼠海马CA3区神经元细胞14d开始得到改善,21d时明显恢复近正常水平,突触结构恢复近正常水平。结论:电针参与海马CA3区神经元突触可塑性的调节,并对抑郁症症状改善起到促进作用。
Objective To explore the effects of electroacupuncture（EA）on behavioral function and synaptic plasticity in hippocampal CA3 area in rats with chronic stress depression.Methods According to the random number table method,144 SD male rats were assigned into a blank group,a model group,an EA group and a fluoxetine group,then each group was divided into a 7dsubgroup,a 14 dsubgroup and a 21 dsubgroup,12rats in each subgroup.The chronic mild unpredictable stress stimulus combined with lonely breeding were applied to establish the depression model of rats,which was performed simultaneously with intervention treatment.The rats in the EA group were treated with EA（dilatational wave）at＂Shenting＂（GV 24）and ＂Baihui＂（GV 20）,while the rats in fluoxetine group were treated with intragastric administration of fluoxetine,once daily.With open-field test,sugar consumption experiment and transmission electron microscope,the changes of behavior and neuronal synapse inhippocampal CA3 area were observed.Results On 7d,14 dand 21d,compared with the blank group,the openfield test score,sugar consumption and body mass were significantly lower in the model group（all P〈0.01）;compared with the model group,the open-field test score,sugar consumption and body mass were significantly higher in the EA group and the fluoxetine group on 14 dand 21d（P〈0.01,P〈0.05）.On 14 dand 21d,compared with the blank group,the synapse in hippocampal CA3 area was significantly lower in the model group（both P〈0.01）;compared with the model group,the synapse in hippocampal CA3 area was significantly higher in the EA group and the fluoxetine group（P〈0.01,P〈0.05）.The neurons cells in hippocampal CA3 area in the model group showed pyknosis and deformation from 7dwith fusion structure and unclear boundary of synapse,which were significantly improved on 21d;the neurons cells in hippocampal CA3 area in the EA group and the fluoxetine group were significantly improved from 14 dand restored to normal level on 21 d,in additio
作者
王珑
张迪
田旭升
刘丹
孙晓伟
李艳秋
王翠娟
马鸣
李冬杰
孙忠人
WANG Long ZHANG Di TIAN Xusheng LIU Dan SUN Xiaowei LI Yanqiu WANG Cuijuan MA Ming LI Dongjie SUN Zhongren(The First Affiliated Hospital of Heilongjiang University of CM, Harbin 150040, China Graduate School of Heilongjiang University of CM Heilongjiang University of CM, Harbin 150040)
出处
《中国针灸》
CAS
CSCD
北大核心
2017年第2期162-168,共7页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金项目:81303044
黑龙江省自然科学基金项目:H2015036
中国博士后科学基金项目:2013M531080
黑龙江省政府博士后基金项目:LBH-Z12252
哈尔滨市科技创新人才专项:2014RFQXJ134
黑龙江中医药大学创新人才支持计划项目资助
中国博士后科学基金特别资助项目:2016T90320
关键词
抑郁
电针
海马CA3
突触可塑性
depression
electroacupuncture（EA）
hippocampal CA3
synaptic plasticity
