摘要
目的 观察电针患侧曲池、阳陵泉穴对脑梗死后肢体痉挛(PSS)大鼠皮质损伤及血清炎性因子[白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)]、谷氨酸(Glu)、丙二醛(MDA)、核黄素激酶(RFK)和还原性辅酶氧化酶2(NOX2)表达的影响,探讨电针治疗缺血性脑卒中后脑损伤的机制。方法 选择SPF级健康成年SD雄性大鼠30只,采用随机数字表法分为假手术组与造模组,每组10、20只。造模组采用Zea-Longa线栓法联合内囊注射N-甲基-D-天冬氨酸(NMDA)制备PSS大鼠模型。造模成功后,造模组按随机数字表法分为模型组、电针组,每组10只。模型组采用鼠板固定大鼠,但不进行干预;电针组接受电针干预,穴位选择患侧阳陵泉穴(左)、曲池穴(左),每穴直刺1针,电针波型:密波;频率:100 Hz,强度以大鼠肢体轻微抖动为度,30 min/次,1次/d,连续治疗7 d。分别于造模第1天和治疗第7天采用Zea-Longa神经功能评分评估大鼠神经功能受损程度;分别于造模第2天和治疗第7天采用改良Ashworth量表(MAS)评分评估大鼠患侧肢体肌张力;采用HE染色观察大脑皮质的病理学改变;行为学测试完毕后,采用酶联免疫吸附法(ELISA法)检测MDA、Glu含量和大脑皮质中IL-6、TNF-α含量;采用Western blot法分析RFK、NOX2蛋白表达水平;采用RT-PCR法分析RFK、NOX2 mRNA转录水平。结果 (1) Zea-Longa神经功能、MAS评分:与治疗前比较,电针组治疗后Zea-Longa神经功能、MAS评分均明显降低(P<0.05)。与假手术组比较,模型组、电针组治疗前Zea-Longa神经功能、MAS评分均明显升高(P<0.05);与模型组比较,电针组治疗后Zea-longa神经功能、MAS评分均明显降低(P<0.05)。(2)脑组织病理学改变:模型组可见脑组织水肿,胞核深染,形态不规则、固缩、空泡变性,炎性细胞浸润等病理学改变;与模型组比较,电针组脑组织病变改善,神经元损伤程度减轻,可见较多正常细胞,细胞轮廓清晰,炎性细胞数�
Objective To observe the effect of electroacupuncture at the affected side of Quchi(LI 11)and Yanglingquan(GB 34)acupoints on cortical injury and serum inflammatory factors[interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)],gluta‐mate(Glu),malondialdehyde(MDA),riboflavin kinase(RFK)and NADPH oxidase 2(NOX2)in rats with post-stroke spasticity(PSS),and to investigate the mechanism of electroacupuncture in alleviating brain injury after ischemic stroke.Methods A total of 30 SPF healthy adult male SD rats were randomly divided into sham operation group and model group,with 10 and 20 rats in each group.The PSS rat model was prepared by Zea-Longa suture method combined with internal capsule injection of N-methyl-D-aspar‐tate(NMDA).After successful modeling,the model group was randomly divided into model group and electroacupuncture group,with 10 cases in each group.The rats in the model group were fixed with the rat plate without intervention,while those in the elec‐troacupuncture group were subjected to electroacupuncture intervention.Yanglingquan(GB34,left)and Quchi(LI 11,left)acupoints on the affected side were selected,and each acupoint was directly punctured with one needle,dense wave pattern,the frequency at 100 Hz,and the intensity based on the slight shaking of rat limbs,30 minutes a time,once a day,and the treatment lasted for 7 days.At the 1st day of modeling and the 7th day of the treatment,Zea-Longa neurological deficit score was used to assess the degree of neurological impairment.At the 2nd day of modeling and the 1st,7th day of intervention,modified Ashworth scale(MAS)was used to assess muscle tension of the affected limbs.Hematoxylin-eosin(HE)staining was used to assess pathological changes of cerebral cortex.Enzyme-linked immunosorbent assay(ELISA)was used to detect the contents of MDA,Glu and IL-6,TNF-αin the cerebral cortex after the behavioral tests were completed.Western blot was used to analyze the protein expression level of RFK and NOX2.RT-PCR was used to analyze mRNA transcription
作者
绽晟
黄麟荇
易丽贞
陈瑞雪
黄慧源
岳增辉
ZHAN Sheng;HUANG Linxing;YI Lizhen;CHEN Ruixue;HUANG Huiyuan;YUE Zenghui(Acupuncture and Moxibustion and Rehabilitation College,Hunan University of Traditional Chinese Medicine,Changsha,Hunan 410208,China)
出处
《康复学报》
CSCD
2024年第2期133-139,共7页
Rehabilitation Medicine
基金
国家自然科学基金项目(81673886)
湖南省自然科学基金项目(2023JJ30462)
湖南省研究生创新课题(CX20220797)。
关键词
脑梗死
肢体痉挛
神经功能障碍
氧化应激
电针
曲池穴
阳陵泉穴
cerebral infarction
limb spasticity
neurological impairment
oxidative stress
electroacupuncture
Quchi(LI 11)acupoint
Yanglingquan(GB34)acupoint
