摘要
目的探析补肾通络方对子宫内膜损伤后人脐静脉内皮细胞模型(human umbilical vein endothelial cells,HUVECs)促增殖、血管生成和迁移作用,及对调控血管内皮生长因子(vascular endothelial growth factor,VEGF)信号通路的影响。方法选择2020年1月—2021年12月,购自武汉大学典型培养物保藏中心的12份HUVECs,给予50 ng/mL VEGF处理后,分别实施20%、10%、5%补肾通络方含药血清和无补肾通络方含药血清干预处理。比较补肾通络方对子宫内膜损伤后,VEGF信号通路的影响及对HUVECs增殖、迁移、血管生成的影响。结果比较20%、10%、5%补肾通络方含药血清和无补肾通络方含药血清干预后24 h的HUVECs活性和24 h细胞生长检测所得OD值,差异无统计学意义(P>0.05);20%补肾通络方含药血清组48 h、72 h的HUVECs活性、细胞生长检测所得OD值及VEGF表达量、HUVECs细胞迁移数均高于10%、5%补肾通络方含药血清组和无补肾通络方血清组,差异有统计学意义(P<0.05)。20%补肾通络方含药血清干预组的血管网状结构完整度优于10%、5%补肾通络方含药血清和无补肾通络方血清,无补肾通络方血清干预组的血管网状结构完整度最差,补肾通络方可逆转VEGF抑制细胞活性的趋势,促进HUVECs的血管形成。结论将补肾通络方应用到子宫内膜损伤中,可逆转VEGF抑制细胞活性的趋势,增强HUVECs的细胞活性,促进细胞增殖、迁移,促进血管新生,促进内皮细胞网络结构的重建,利于促进内皮细胞损伤的修复,利于子宫内膜功能的恢复,且与补肾通络方含药血清浓度呈一定量效关系。
Objective To investigate the effects of Bushen Tongluo Recipe(补肾通络方)on the proliferation,angiogenesis and migration of human umbilical vein endothelial cells(HUVECs)after endometrial injury,as well as the effect on the regulation of vascular endothelial growth factor(VEGF)signaling pathway.Methods The experiment selected 12 HUVECs purchased from Wuhan University Type Culture Collection Center from January 2020 to December 2021.After treated with 50 ng/mL VEGF,20%,10% and 5% Bushen Tongluo Recipe-containing serum and no Bushen Tongluo Recipe-containing serum were carried out respectively.The effects of Bushen Tongluo Recipe on VEGF signaling pathway after endometrial injury and its effects on the proliferation,migration and angiogenesis of HUVECs were compared.Results Comparing 20%,10% and 5% Bushen Tongluo Recipe-containing serum and no Bushen Tongluo Recipe-containing serum at 24 h after intervention,the activity of HUVECs and the OD value obtained by 24 h cell growth detection had no statistical significance(P>0.05).The 20% Bushen Tongluo Recipe-containing serum group had higher HUVECs activity at 48 h and 72 h,OD value and VEGF expression from cell growth detection,and HUVECs cell migration number was higher than that of the 10% and 5% Bushen Tongluo Recipe-containing serum groups and no Bushen Tongluo Recipe-containing serum group,and the difference was statistically significant(P<0.05).The integrity of the vascular network structure in the 20% Bushen Tongluo Recipe-containing serum intervention group was better than that of the 10% and 5% Bushen Tongluo Recipe-containing serum groups and no Bushen Tongluo Recipe-containing serum group,and the integrity of the network structure was the worst in the blood vessels of the no Bushen Tongluo Recipe-containing serum group.Bushen Tongluo Recipe can reverse the tendency of VEGF to inhibit cell activity and promote the angiogenesis of HUVECs.Conclusion The application of Bushen Tongluo Recipe to endometrial injury can reverse the tendency of VEGF to inhibit
作者
秦屹
樊利杰
任淼
李萍
吕改琴
郭亚楠
QIN Yi;FAN Lijie;REN Miao;LI Ping;LYU Gaiqin;GUO Ya'nan(China Rehabilitation Research Center,Beijing 100068,China;The Seventh Medical Center,PLA General Hospital,Beijing 100700,China;The Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100700,China)
出处
《辽宁中医杂志》
CAS
北大核心
2024年第4期153-157,共5页
Liaoning Journal of Traditional Chinese Medicine
基金
北京中医药大学新教师启动基金项目(2021-BUCMXJKY012)。
关键词
子宫内膜损伤
补肾通络方
人脐静脉内皮细胞
血管内皮生长因子
血管生成
endometrial injury
Bushen Tongluo Recipe(补肾通络方)
human umbilical vein endothelial cells
vascular endothelial growth factor
angiogenesis
