摘要
为探究湖北襄阳地区高温大曲中芽孢杆菌(Bacillus)菌群多样性,该研究通过下载并筛选高温大曲样品芽孢杆菌相对含量>2.0%的测序数据,采用Illumina Mi Seq高通量测序技术和传统纯培养技术相结合的方法对大曲样品中芽孢杆菌多样性进行解析。结果表明,高温大曲中芽孢杆菌相对含量>2.0%的样品共有12份,包括8份白色大曲样品和4份黄色大曲样品,所有样品共获得77 825条代表性序列,1 197个操作分类单元(OTUs)。多样性分析结果表明,较之黄色大曲,白色大曲样品中芽孢杆菌的超1指数和发现物种数显著偏高(P<0.05)。OTU分析结果表明,OTU5321和OTU5291在所有样品中均存在,其在白色大曲和黄色大曲中累积包含序列分别占总序列数的49.43%和41.32%。传统纯培养技术分析结果表明,解淀粉芽孢杆菌(Bacillus amyloliquefaciens)为高温大曲中芽孢杆菌的主要可培养菌种。由此可知,白色大曲芽孢杆菌群落结构的丰富度更高,且解淀粉芽孢杆菌为高温大曲中芽孢杆菌的主要可培养菌种,为后续高温大曲中芽孢杆菌的应用及菌种选育具有指导作用。
In order to explore the diversity of Bacillus in high-temperature Daqu in Xiangyang of Hubei province,the sequencing data with Bacillus relative content>2.0%of high-temperature Daqu samples were downloaded and screened.The diversity of Bacillus in Daqu samples was analyzed by Illumina MiSeq high-throughput sequencing technology combined with traditional pure culture technology.The results showed that there were 12 samples with Bacillus content>2.0%in high-temperature Daqu,including 8 white Daqu samples and 4 yellow Daqu samples.A total of 77825 representative sequences and 1197 operational taxonomic units(OTUs)were obtained from all samples.Diversity analysis showed that compared with yellow Daqu,the Chao 1 index and discovered species number of Bacillus in white Daqu samples were significantly higher(P<0.05).OTU analysis showed that OTU5321 and OTU5291 were present in all samples,and the cumulative inclusion sequences in white and yellow Daqu accounted for 49.43%and 41.32%of the total sequences number,respectively.The results of traditional pure culture technology analysis showed that Bacillus amyloliquefaciens was the main cultivable strain of Bacillus in high-temperature Daqu.In conclusion,the richness of Bacillus community structure in white Daqu was higher,and B.amyloliquefaciens was the main culturable strain of Bacillus in high temperature Daqu,which had guiding effect on the application and strain selection of Bacillus in high-temperature Daqu.
作者
黄锶钘
王玉荣
贺子豪
田龙新
周加平
刘菊珍
郭壮
HUANG Sixing;WANG Yurong;HE Zihao;TIAN Longxin;ZHOU Jiapin;LIU Juzhen;GUO Zhuang(Hubei Provincial Engineering and Technology Research Center for Food Ingredients,Hubei University of Arts and Science,Xiangyang 441053,China;Xiangyang Jiangxiang Baijiu Solid State Fermentation Enterprise-School Joint Innovation Center,Xiangyang 441053,China;Xiangyang Key Laboratory of Solid State Fermentation of Jiangxiang Baijiu,Xiangyang 441614,China)
出处
《中国酿造》
CAS
北大核心
2024年第3期79-83,共5页
China Brewing
基金
湖北省自然科学基金计划襄阳创新发展联合基金项目(2023AFD049)
湖北文理学院教师科研能力培育基金“科技创新团队”(2020kypytd009)
湖北文理学院科研启动基金(kyqdf2021014)。
关键词
高温大曲
芽孢杆菌
高通量测序
操作分类单元
传统纯培养技术
high-temperature Daqu
Bacillus
high throughput sequencing
operational taxonomic unit
traditional pure culture technology
