摘要
目的:分析北京市昌平区2018—2019年6起副溶血性弧菌食源性疾病聚集事件分离株的血清型、耐药性、毒力基因和分子特征。方法:对6起事件中的23株副溶血性弧菌进行血清学分型、毒力基因检测、微量肉汤稀释药敏实验和脉冲场凝胶电泳。结果:21株人源分离株鉴定为3种血清型(O3:K6、O4:K8和O4:KUT),主要为O3:K6型,毒力基因均为tlh^(+)、tdh^(+)、trh^(-);2株食品分离株血清型为O8:K20,毒力基因为tlh^(+)、tdh^(-)、trh^(-)。药敏结果显示,对头孢唑啉12株菌敏感,5株耐药,6株中介;对氨苄西林1株菌中介,其余菌株敏感;对其他15种抗生素23株菌均敏感。PFGE聚类分析显示,23株副溶血性弧菌分为8种PFGE带型,5起事件的人源分离株属于优势PFGE带型PT06。结论:6起事件中的副溶血性弧菌血清型主要为O3:K6,多数菌株属于优势带型PT06,无多重耐药菌出现,人源分离株均携带毒力基因tlh和tdh。
Objective:To analyze the serotype,drug resistance,virulence genes and PFGE characteristics of strains isolated from 6 Vibrio parahaemolyticus food poisoning incidents in Changping district of Beijing in 2018—2019.Method:Serotyping,virulent genes detection,broth microdilution method and pulsed field gel electrophoresis(PFGE) typing tests were performed on 23 Vibrio parahaemolyticus strains in 6 incidents.Result:The 21 human isolated strains were identified as three serotypes(O3:K6,O4:K8,and O4:KUT),O3:K6 was the dominant serotype,and the virulence genes were tlh^(+),tdh^(+),trh^(-).The 2 strains collected from food samples were O8:K20,and the virulence genes were tlh^(+),tdh^(-),trh^(-).12 strains are sensitive to cefazolin,5 strains are resistant,and 6 strains are intermediate.There was 1 strain showed intermediate to ampicillin,while all strains were sensitive to the other 15 antibiotics.The 23 strains of Vibrio parahaemolyticus were analyzed by PFGE clustering to obtain 8 PFGE patterns,the pandemic pattern PT06 was found in 5 incidents.Conclusion:The dominant serotypes of Vibrio parahaemolyticus strains isolated from 6 incidents were O3:K6 and the predominant PFGE patterns was PT06.There were no multiantibiotic resistance.All human isolated strains had the virulence gene tlh and tdh.
作者
李东迅
舒高林
王维钧
张阳
谷田
牛桓彩
LI Dongxun;SHU Gaolin;WANG Weijun;ZHANG Yang;GU Tian;NIU Huancai(Changping District Center for Disease Control and Prevention,Beijing 102206,China)
出处
《食品安全导刊》
2023年第18期89-91,98,共4页
China Food Safety Magazine
关键词
副溶血性弧菌
血清型
脉冲场凝胶电泳
耐药性
毒力基因
Vibrio parahaemolyticus
serotypes
pulsed field gel electrophoresis
drug resistance
virulence genes
