摘要
试验旨在研究小麦胚芽肽和大豆酶解蛋白的分子质量分布和体外抗氧化活性。试验采用单因素完全随机设计,以维生素C为对照,分别测定小麦胚芽肽和大豆酶解蛋白在0.625、1.25、2.5、5、10 mg/mL浓度下对DPPH自由基、羟自由基和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基的清除率,并通过测定T-AOC吸光度值计算总还原力。结果表明:使用高效液相色谱测定小麦胚芽肽和大豆酶解蛋白重均分子质量分别为513 u和551 u,二者分子质量小于1000 u的比例分别为90.22%和86.93%。在一定浓度范围内,随小麦胚芽肽和大豆酶解蛋白浓度的升高,其体外抗氧化性能线性增加(P<0.05),且呈正相关关系。在10 mg/mL浓度时,小麦胚芽肽对DPPH自由基、羟自由基和ABTS自由基清除率和总还原力分别为90.39%、70.66%、96.24%和2.23,大豆酶解蛋白对DPPH自由基、羟自由基和ABTS自由基清除率和总还原力分别达到65.55%、68.21%、75.14%和0.51。综上,在本试验条件下,不同浓度小麦胚芽肽和大豆酶解蛋白均具有一定的抗氧化能力,且小麦胚芽肽相较于大豆酶解蛋白的抗氧化能力更强。
This experiment was conducted to study the molecular weight distribution and the in vitro anti-oxidant properties of wheat germ peptides and soybean protein hydrolysate.In this single-factor experi-mental design,taking vitamin C as the control,the scavenging rates of wheat germ peptide and soybean protein hydrolysate on DPPH free radicals,·OH free radicals and ABTS free radicals were measured at different concentrations(0.625,1.25,2.5,5,10 mg/mL)by salicylic acid method,DPPH method and ABTS method,and calculate the total reduction force by measuring the absorbance value of T-AOC.The results showed that the average molecular weight of wheat germ peptide and soybean protein hydro-lysate was 513 u and 551 u determined by HPLC,and the percentage of oligopeptide with molecular weight less than 1000 u was 90.22%and 86.93%,respectively.The in vitro antioxidant properties of wheat germ peptide and soybean enzyme digested protein increased linearly with the increase of their concentrations in a certain concentration range(P<0.05),which showed a positive correlation.At a con-centration of 10 mg/mL,the scavenging rate of wheat germ peptide on DPPH radicals,hydroxyl radicals and ABTS radicals were 90.39%,70.66%,96.24%and 2.23;the scavenging rate and total reducing power of DPPH radicals,hydroxyl radicals and ABTS radicals reached 65.55%,68.21%,75.14%and 0.51,respectively,for soybean enzymatic pro-tein at 10 mg/mL.In conclusion,under the condi-tions of this experiment,both wheat germ peptide and soybean enzymatic protein at different concen-trations have antioxidant capacity,and the antioxi-dant capacity of wheat germ peptide was higher than that of soybean enzymatic protein.
作者
边智尧
陈宝江
申帅峰
随腾玖
韩帅娟
BIAN Zhiyao;CHEN Baojiang;SHEN Shuaifeng;SUI Tengjiu;HAN Shuaijuan(College of Animal Science and Technology,Hebei Agricultural University,Hebei Baoding 071000,China)
出处
《饲料工业》
CAS
北大核心
2024年第7期80-86,共7页
Feed Industry
基金
河北省重点研发计划项目[21326606D]
河北省省属高等学校基本科研业务费研究项目[KY202032]。
关键词
小麦胚芽肽
大豆酶解蛋白
体外抗氧化性能
总还原力
自由基清除率
wheat germ peptide
soybean protein hydrolysate
in vitro antioxidant properties
total reduc-ing power
free radical scavenging rate
