摘要
目的:探讨妊娠期高血压疾病(HDP)患者血清circACTR2、miR-525-5p水平与不良妊娠结局的相关性。方法:选取2020年1月-2022年6月在本院建档并定期产前检查的HDP患者148例为HDP组,产前检查正常并分娩的100例孕妇作为对照组,收集临床资料,根据是否发生不良妊娠结局将HDP患者分为正常妊娠结局组98例、不良妊娠结局组50例。采用实时荧光定量PCR法检测孕妇血清circACTR2和miR-525-5p表达水平;分析HDP患者妊娠结局与临床病理特征关系;采用Spearman法分析HDP患者血清circACTR2和miR-525-5p表达与不良妊娠结局的相关性;采用logisitic回归分析HDP患者不良妊娠结局的影响因素;采用受试者工作特征(ROC)曲线分析HDP患者血清circACTR2、miR-525-5p表达预测不良妊娠结局价值。结果:HDP组血清circACTR2(1.33±0.35)高于对照组(1.08±0.27),miR-525-5p(0.76±0.21)低于对照组(1.02±0.30)(P<0.05);HDP不良妊娠结局患者血清胱抑素C(CysC)、D-二聚体(D-D)、C反应蛋白(CRP)和circACTR2水平高于HDP正常妊娠结局患者,miR-525-5p水平低于HDP正常妊娠结局患者(P<0.05);血清circACTR2水平与不良妊娠结局呈正相关,血清miR-525-5p水平与不良妊娠结局呈负相关(均P<0.05);CysC、D-D、CRP、circACTR2和miR-525-5p异常均为HDP患者不良妊娠结局的影响因素(P<0.05);circACTR2联合miR-525-5p预测HDP患者不良妊娠结局的曲线下面积为0.877,敏感度76.0%,特异度86.7%。结论:HDP患者血清circACTR2表达上调,miR-525-5p表达下调,二者与HDP不良妊娠结局密切相关,二者联合检测对预测HDP患者不良妊娠结局有较好效能。
Objective:To investigate the correlation between the serum circACTR2 and miR-525-5p levels of pregnant women with hypertensive disorder of pregnancy(HDP)and their adverse pregnancy outcomes.Methods:From January 2020 to June 2022,148 pregnant women with HDP who were filed in hospital and underwent regular prenatal examination were selected in study group,and 100 healthy pregnant women who underwent regular prenatal examination and gave birth in hospital during the same period were selected in control group.The clinical data of the women in the two groups were collected.The women in the two groups were divided into group A(98 women with the normal pregnancy outcomes)and group B(50 women with the adverse pregnancy outcomes)according to whether the adverse pregnancy outcome occurred or not.The expression levels of serum circACTR2 and miR-525-5p of the women in the two groups were detected by real-time fluorescent quantitative PCR.The correlation between the pregnancy outcomes of the women with HDP and their clinicopathological features was analyzed.Spearman method was used to analyze the correlation between the expressions of serum circACTR2and miR-525-5p of the women with HDP and their adverse pregnancy outcomes.Logisitic regression was used to analyze the influencing factors of the adverse pregnancy outcomes of the women with HDP.Receiver operating characteristic(ROC)curve was used to analyze the value of the serum circACTR2and miR-525-5p expressions of the women for predicting their adverse pregnancy outcomes.Results:The serum circACTR2level(1.33±0.35)of the women in the study group was significantly higher than that(1.08±0.27)of the women in the control group,and the miR-525-5p level(0.76±0.21)of the women in the study group was significantly lower than that(1.02±0.30)of the women in the control group(P<0.05).The levels of serum cystatin C(CysC),D-dimer(D-D),C-reactive protein(CRP)and circACTR2of the women in group B were significantly higher than those of the women in group A,and the level of miR-525-5p of t
作者
张昌清
梅茹
ZHANG Changqing;MEI Ru(Shiyan Maternal and Child Health Care Hospital,Shiyan,Hubei Province,442000)
出处
《中国计划生育学杂志》
2024年第3期660-664,668,共6页
Chinese Journal of Family Planning
基金
湖北省卫生健康委科研项目(WJ2023F091)。
