摘要
目的 了解河北省卢龙县5岁以下儿童病毒性腹泻的病原组成及变化趋势,为婴幼儿病毒性腹泻防治提供科学依据。方法 收集卢龙县某医院和某妇幼保健院2016年1月1日-2021年12月31日共832份5岁以下住院患儿粪便标本,并填写流行病学个案调查表。轮状病毒(Rotavirus, RV)抗原及其阳性标本G/P进一步分型检测分别用酶联免疫吸附试验(enzyme linked immunosorbent assay, ELISA)和逆转录聚合酶链式反应(reverse transcription PCR,RT-PCR)。人类杯状病毒(Human calicivirus, HuC V)、肠道腺病毒(Enteric adenovirus, EAdV)、星状病毒(Astrovirus,AstV)用RT-PCR或实时荧光定量PCR方法检测,诺如病毒(Norovirus, NV)阳性样本经RT-PCR扩增后测序分型。结果 832份标本共检出4种病毒576份,总阳性检出率为69.23%, RV、 HuC V、 EAdV和AstV检出率分别为38.22%、 31.01%、 6.01%和5.17%,单一病原体感染检出率为58.41%,混合感染检出率为10.82%,其中2种病毒合并感染的样本87份, 3种病毒合并感染的样本3份。不同性别间各病毒及混合感染阳性检出率差异无统计学意义(χ^(2)_(RV)=0.811,PRV=0.368;χ^(2)_(HuCV)=0.286,PHuCV=0.593;χ^(2)_(EAdV)=0.156,PEAdV=0.693;χ^(2)AstV=0.035,PAstV=0.852;χ混合感染2=0.617,P混合感染=0.432)。RV和HuC V以18~<24月龄阳性检出率最高(χ^(2)_(RV)=18.748,PRV=0.009;χ^(2)_(HuCV)=2.797,PHuCV=0.903),分别为47.69%和33.85%。病毒性腹泻在1~3月和11、 12月为高发季, RV在2月阳性检出率最高(59.51%), HuC V在11月阳性检出率最高(62.90%), HuC V和EAdV性腹泻全年均有发病。RV中G9P[8]型占90.25%, HuC V中GⅡ型占67.05%。结论 卢龙县5岁以下婴幼儿病毒性腹泻病原以RV和HuC V为主,HuC V阳性检出率增幅较大。RV以G9P[8]型、 HuC V以GⅡ型为主要流行株。
Objective To understand the pathogen composition and changing trend of viral diarrhea in children under 5 years old in Lulong County,Hebei Province,so as to provide scientific basis for the prevention and treatment of viral diarrhea in infants and young children.Methods A total of 832 stool samples of children under 5 years old were collected from a Lulong County hospital and maternal and child health hospital from January 1,2016 to December 31,2021,and the epidemiological case study form was filled in. Rotavirus( RV) antigen and its positive G/P samples were further typing by enzyme linked immunosorbent assay(ELISA) and reverse transcription PCR(RT-PCR),respectively. Human calicivirus(Hu CV),enteric Adenovirus(EAd V) and Astrovirus(Ast V) were detected by RT-PCR or real-time quantitative PCR. Norovirus(NV) positive samples were amplified by RT-PCR and then sequenced and typed.Results A total of 576 of 832 samples were positive for four kinds of viruses,and the total positive detection rate was 69. 23%. The positive detection rates of RV,Hu CV,EAd V and Ast V were 38. 22%,31. 01%,6. 01% and 5. 17%,respectively. Single pathogen infection detection rate was 58. 41%,and mixed infection detection rate was 10. 82%,of which 87 samples were co-infected with two viruses and 3 samples were co-infected with three viruses. There was no statistically significant difference in the positive detection rates of viruses and mixed infections between different genders(χ^(2)_(RV)= 0. 811,P-(RV)= 0. 368;χ^(2)_(HuCV)= 0. 286,P_(HuCV)= 0. 593;χ^(2)_(EAdV)= 0. 156,P_(EAdV)= 0. 693;χ_(AstV)~2= 0. 035,P_(AstV)= 0. 852;χ_(mixed infection)= 0. 617,P_(mixed infection)= 0. 432). RV and Hu CV had the highest positive detection rates of 47. 69% and 33. 85% at the age of 18 to less than 24 months(χ^(2)_(RV)= 18. 748,P_(RV)= 0. 009;χ^(2)_(HuCV)= 2. 797,P_(HuCV)= 0. 903),respectively. The peak seasons of viral diarrhea were January to March,November and December. RV had the highest positive detection rate in February(59. 51%),and Hu CV had
作者
闫晓凤
于秋丽
刘莹莹
谢赟
赵文娜
邸学渊
郝玉军
YAN Xiaofeng;YU Qiuli;LIU Yingying;XIE Yun;ZHAO Wenna;DI Xueyuan;HAO Yujun(Hebei Lulong Center for Disease Control and Prevention,Qinhuangdao,Hebei 066400,China;不详)
出处
《医学动物防制》
2024年第3期234-239,共6页
Journal of Medical Pest Control
基金
河北省医学科学研究重点课题(20160059)
河北省医学科学研究课题(20200225)。
关键词
卢龙县
婴幼儿
病毒性腹泻
病原分析
Lulong County
Infants and young children
Viral diarrhea
Etiologic analysis
