摘要
本研究以兰州苦水玫瑰为原料,采用超声微波协同萃取法提取玫瑰精油,以精油提取率为指标,研究超声微波时间、超声微波功率、料液比、氯化钠质量浓度4个因素对提取率的影响,在单因素的基础上采用Box-Behnken响应面法优化提取工艺参数,结果表明:在料液比1∶16 g/mL,溶剂氯化钠质量浓度4.1 g/L,超声波50 W,微波功349 W条件下预处理127 s,精油提取率最高,可达到1.274%。体外抑菌结果表明,玫瑰精油对金葡萄球菌和大肠杆菌均呈现不同程度的抑制作用,抑菌圈直径分别为(13.53±0.21)、(11.45±0.18)mm,其中对金葡萄球菌的最小抑菌浓度(MIC)为(1.25±0.01)μL/mL,对大肠杆菌的最小抑菌浓度(MIC)为(0.625±0.030)μL/mL,表明兰州苦水玫瑰精油在食源性抑菌剂及化妆品领域具有巨大的开发潜力。
In this study,Lanzhou kushui rose was used as the raw material to extract rose essential oil by ultrasonic microwave synergistic extraction method,the influence of four factors,including ultrasonic microwave time,ultrasonic microwave power,material-liquid ratio,and sodium chloride concentration,on the extraction rate was investigated,and the Box-Behnken response surface method was used to optimize the extraction process parameters,The results indicated that the highest essential oil extraction rate of 1.274%could be achieved under the pretreatment conditions as follows:the material-liquid ratio of 1∶16 g/mL,the solvent concentration of sodium chloride of 4.1 g/L,ultrasonic waves of 50 W,and microwave power was of W for 127 s.The rose essential oil also showed good antimicrobial activities against Staphylococcus aureus and Escherichia coli,and the diameter of the inhibition circle was(13.53±0.21)mm and(11.45±0.18)mm,respectively,where the minimum inhibitory concentration(MIC)for Staphylococcus aureus was(1.25±0.01)μL/mL,and the minimum inhibitory concentration(MIC)for Escherichia coli was(0.625±0.030)μL/mL,indicating that Lanzhou kushui rose essential oil has great potential for development in the field of food-borne antibacterial agents and cosmetics.
作者
马金璞
施生玲
张钰璇
郭星晨
蔡勇
郭鹏辉
杨具田
高丹丹
Ma Jinpu;Shi Shengling;Zhang Yuxuan;Guo Xingchen;Cai Yong;Guo Penghui;Yang Jutian;Gao Dandan(China-Malaysia National Joint Laboratory,Biomedical Research Center,Northwest Minzu University ,Lanzhou 730030;College of Life Science and Engineering,Northwest Minzu University ,Lanzhou 730124)
出处
《中国粮油学报》
CAS
CSCD
北大核心
2023年第12期151-157,共7页
Journal of the Chinese Cereals and Oils Association
基金
国家自然科学基金计划项目(31960461),西北民族大学中央高校基本科研业务费专项(31920220057、BELTY201901),兰州市城关区科技计划项目(2022JSCX0011),西北民族大学大学生创新创业训练计划项目(X202210742292)。
关键词
兰州苦水玫瑰
精油
超声微波
响应面优化
抑菌
Lanzhou kushui rose
essential oil
ultrasonic microwave
box-behnken
bacteriostatic
