摘要
近年来,精液检测技术在评估男性生育力方面取得了显著进展,为临床诊断和治疗提供了先进的工具.这些技术包括临床常规的精液检测技术,如精液常规分析、计算机辅助精子分析和DNA碎片检测等.然而,由于干扰因素的复杂性,目前的方法尚无法全面评估生育力以及缺乏明确的病因学检测方案等问题,需要进一步开发用于精子检测及男性生育力评估的技术.目前,现代检测技术中逐步使用电子显微镜、高速运动成像分析、流式细胞仪及激光共聚焦等仪器工程,为精子的质量评估提供了更全面准确的信息.未来,随着技术的不断进步,将会出现更加精准和高效的精液检测技术.睾丸类器官技术将使男性生育力评估更为精确与全面;荧光活体成像技术可以实现对精子内指标的活体无创观测,使得临床病因学检测更加高效、便捷;基于组学测序技术的精子分析方法,可以实现对精子源性遗传突变的全面评估,包括基因组学、表观遗传学和蛋白质组学等方面的信息;人工智能和机器学习的应用也有望提高精子检测的准确性和效率,为临床决策提供更可靠的依据.这些先进的精液检测技术的发展将为不育症患者提供更准确的诊断和个体化的治疗方案.同时,它们也有助于深入了解男性生育力的机制和疾病发生的分子基础,为生殖医学领域的研究和发展提供重要的支持.
In recent years,significant advancements have been made in sperm detection techniques for assessing male fertility,providing advanced tools for clinical diagnosis and treatment.These techniques include conventional methods such as semen analysis,computer-assisted sperm analysis,and DNA fragmentation detection.However,further development of technologies for sperm detection and male fertility assessment is needed due to the complexity of interfering factors,the inability of current methods to comprehensively evaluate fertility,and the lack of clear etiological testing protocols.Modern detection technologies,such as electron microscopy,high-speed motion imaging analysis,flow cytometry,and laser confocal microscopy,have gradually been employed to provide more comprehensive and accurate information for assessing sperm quality.In the future,with continuous technological advancements,more precise and efficient sperm detection techniques are expected to emerge.Testicular organ engineering will enable more accurate and comprehensive assessment of male fertility.Fluorescence live imaging techniques can achieve non-invasive observation of intracellular sperm indicators,making clinical etiological testing more efficient and convenient.Sperm analysis methods based on genomic sequencing technologies can comprehensively evaluate sperm-originated genetic mutations,including information from genomics,epigenetics,and proteomics.The application of artificial intelligence and machine learning is also expected to improve the accuracy and efficiency of sperm detection,providing more reliable evidence for clinical decision-making.The development of these advanced sperm detection techniques will provide more accurate diagnoses and personalized treatment plans for infertility patients.Moreover,they will contribute to a deeper understanding of the mechanisms underlying male fertility and the molecular basis of reproductive disorders,providing crucial support for research and development in the field of reproductive medicine.
作者
吴思娴
魏亚祯
于抗抗
陈雨晞
许文明
WU SiXian;WEI YaZhen;YU KangKang;CHEN Yuxi;XU WenMing(Key Laboratory of Birth Defects and Related Diseases of Women and Children(Sichuan University),Ministry of Education,West China Second University Hospital,SichuanUniversity,Chengdu 610041,China;West China School of Pharmacy,Sichuan University,Chengdu 610041,China;Collegeof Life Sciences,Sichuan University,Chengdu 610064,China)
出处
《中国科学：生命科学》
CSCD
北大核心
2024年第1期173-186,共14页
Scientia Sinica(Vitae)
基金
国家自然科学基金(批准号:82171715)资助。
关键词
精液检测
男性生育力
类器官
人工智能
先进技术
sperm detection
male fertility
organoid
artificial intelligence
advanced technology
