摘要
试验以竹燕窝(海绵胶煤炱菌)为材料开发新型饲料添加剂。采用正交试验确定竹燕窝提取物的最佳提取工艺条件。采用牛津杯法探讨竹燕窝乙醇提取物对3种革兰氏阳性菌[粪肠球菌(Enterococcus faecalis)、金黄色葡萄球菌(Staphylococcus aureus)、表皮葡萄球菌(Staphylococcus epidermidis)]和3种革兰氏阴性菌[鼠伤寒沙门氏菌(Salmonella typhimurium)、鸡沙门氏菌(Salmonella pullorum)、大肠杆菌(Escherichia coli)]的抑菌效果。结果表明,竹燕窝抑菌提取物的最优提取工艺为乙醇浓度85%、液料比5 mL/g、提取时间180 min、提取温度50℃。竹燕窝乙醇提取物对革兰氏阳性菌的抑菌效果优于革兰氏阴性菌。研究表明,试验明确了竹燕窝在抑菌方面的潜在应用价值,可为抑菌产品的开发提供参考。
The experiment used Scorias spongiosa as the material,to develop a new limiting resistance feed additive.An orthogonal test was used to determine the optimum extraction conditions of Scorias spongiosa extract.The Oxford cup method was employed to investigate the antibacterial effects of ethanol extracts from Scorias spongiosa on three Grampositive bacteria(Enterococcus faecalis,Staphylococcus aureus,and Staphylococcus epidermidis)and three Gramnegative bacteria(Salmonella typhimurium,Salmonella pullorum,and Escherichia coli).The results indicated that the optimal extraction process for Scorias spongiosa inhibitory extracts was an ethanol concentration of 85%,a liquid-tomaterial ratio of 5 mL/g,an extraction time of 180 minutes,and an extraction temperature of 50℃.The inhibitory effect of Scorias spongiosa ethanol extracts on Gram-positive bacteria was superior to that on Gram-negative bacteria.The study clarifies the potential application value of Scorias spongiosa in terms of antibacterial properties,providing reference for the development of antibacterial products.
作者
冯鹏
俞骏晨
王易鑫
贾玮
苏洋
汪瀚宇
廖菲
农向
FENG Peng;YU Jun-chen;WANG Yi-xin;JIA Wei;SU Yang;WANG Han-yu;LIAO Fei;NONG Xiang
出处
《饲料研究》
CAS
北大核心
2024年第1期80-85,共6页
Feed Research
基金
乐山师范学院校级高层次人才启动项目(项目编号:RC2021013)
乐山师范学院校级科研培育项目重点项目(项目编号:KYPY2024-0005)
乐山师范学院校级科研培育项目(项目编号:2022SSDJS009)
2022年乐山师范学院创新创业学院资助项目(项目编号:SC-DGZZ202211)
乐山市科技局重点项目(项目编号:22ZDYJ0090)
2022年大学生创新创业训练计划项目(项目编号:S202210649156)。
关键词
竹燕窝
革兰氏阳性菌
革兰氏阴性菌
抑菌活性
Scorias spongiosa
Gram-positive bacteria
Gram-negative bacteria
antibacterial activity
