摘要
目的:建立纵条纹炭角菌菌丝体液体培养的最佳条件。方法:以菌丝体生物量为指标,采用单因素试验及正交优化试验,统计学方法确定纵条纹炭角菌液体培养的最佳条件。结果:单因素实验表明,碳源以麦芽糖、葡萄糖或玉米粉最佳,最佳氮源为黄豆饼粉,最佳碳氮比为5∶1,其他最佳培养参数为pH 6、接种2块菌饼、振荡培养7 d。K、Mg、P及VB1对菌丝体生长有明显的促进作用。正交试验表明,在麦芽糖4%、黄豆饼粉0.8%、pH 7、25℃及振荡培养13 d的条件下,菌丝体生物量最大。结论:液体培养优化条件下可大量获得纵条纹炭角菌菌丝体,菌丝体干重可达8.65 g/L以上。
Objective：To establish the optimum submerged culture condition of Xylaria striata mycelium. Methods：One-factor-at-atime method and orthogonal experiment design were applied and the dry weight of mycelium was tested for evaluation the biomass of the growth of Xylaria striata mycelium. Results：The results from one-factor-at-a-time experiments showed that maltose,glucose or corn powder could be used as the best carbon source and the optimum nitrogen source was soybean powder. The best ratio of carbon source to nitrogen source was 5∶ 1. In addition,the higher dry weight of mycelium was found at pH 6 when 2 mycelial discs were inoculated and fermented for 7 days. Otherwise,the growth of mycelium was observed to be promoted significantly by addition with K,Mg,P and VB1. The consequence of orthogonal experiment showed that the optimum carbon source and nitrogen source were maltose 4% and soybean powder0. 8%,respectively,and the highest mycelium biomass could be obtained at pH 7 and 25 ℃ shaking for 13 days. Conclusion：A large amount of mycelium will be obtained under the optimum condition of liquid culture for Xylaria striata.
出处
《中药材》
CAS
CSCD
北大核心
2014年第8期1317-1321,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(21272189)
关键词
纵条纹炭角菌
液体培养
单因素试验
正交试验
Xylaria striata Pat.
Liquid fermentation
One-factor-at-a-time method
Orthogonal experiment
