摘要
旨在研究1-甲基海因(1-methylhydantoin,MH)对钠离子通道(Nav channels,Navs)1.8的干预作用,为临床科学治疗疼痛提供理论依据,为实现“疼痛最小化”终极目标拓宽途径。选取60只SPF级SD大鼠通过随机分组设计,各组分别使用1-甲基海因(MH组)、利多卡因(L组)、氨溴索(A组)处理,第21和28天使用蛋白免疫印记法检测大鼠L4~L6段背根神经节(dorsal root ganglia,DRG)Nav1.8的蛋白表达,采用全细胞膜片钳技术检测MH作用下的Nav1.8通道峰电流。结果表明,与M组相比,MH能使Nav1.8表达显著下降(P<0.05)。MH对Nav1.8通道峰电流具有一定抑制作用,10和100μmol·L^(-1)MH对Nav1.8的电流抑制率分别为6.34%±3.13%和14.6%±1.52%,具有量效关系。提示MH能够下调慢性疼痛大鼠DRG中Nav1.8的表达,同时抑制Nav1.8电流,进而产生镇痛作用。
The purpose of this study is to investigate the effect of 1-methylhydantoin on Nav Channels 1.8,which can provide a theoretical basis for pain intervened and different ways to a-chieve the ultimate goal of"pain minimization".Sixty SPF SD rats were randomly divided into six groups.The group was treated with 1-methylhyne(group MH),lidocaine(group L)and am-broxol(group A),respectively.On the 21st and 28th day after modeling,Western blot was used to detect the protein expressions of Nav1.8 in the L4-L6 dorsal root ganglia of rats,and patch-clamp technique was used to detect the peak currents of Nav1.8 channels under the effect of MH.Comparing with the group of model,the results showed that MH could decrease the expression of Nav1.8 significantly(P<0.05).MH inhibited the channel peak currents of Nav1.8 to a certain extent.The current inhibition rates of 10μmol·L^(-1)and 100μmol·L^(-1)MH to Nav1.8 were 6.34%±3.13%and 14.6%±1.52%respectively,which is showing a dose-response relation-ship.It is suggested that MH can decrease the expression of Nav1.8 in DRG of rats with chronic pain,and inhibit Nav1.8 current at the same time,thus producing analgesic.
作者
梁雾滢
刘镇
曾玉淇
吕俊瑾
莫睿文
远立国
LIANG Wuying;LIU Zhen;ZENG Yuqi;LÜJunjin;MO Ruiwen;YUAN Liguo(College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases,Guangzhou 510642,China;Guangdong Technological Engineering Research Center for Pets,Guangzhou 510642,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2023年第12期5312-5317,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
广东省自然科学基金(2013B040200032)。
