摘要
目的 探讨部分神经损伤(spared nerve injury,SNI)致神经病理性疼痛过程中背根神经节(dorsal root ganglion,DRG)内Nav1.7、Nav1.8以及交感神经芽生之间的关系。方法 将实验大鼠随机分为对照组(Control组,n=18)和手术组(SNI组,n=18),于术后第3、7、14、21天测机械痛阈(mechanical withdrawal threshold,MWT),于术后7天和21天取术侧腰段DRG,分别检测Nav1.7、Nav1.8、生长相关蛋白43(growth-associated protein 43,GAP43)、酪氨酸羟化酶(tyrosine hydroxylase,TH)的mRNA表达及蛋白含量,并且进行荧光染色。结果 与对照组相比,SNI组MWT降低,且呈时间依赖性。SNI术后7天DRG中Nav1.7、Nav1.8、GAP43和TH的mRNA表达和蛋白含量同步增加。免疫荧光半定量发现Nav1.7、Nav1.8、GAP43在SNI术后21天中大直径神经元异常表达增多。TH与Nav1.7、Nav1.8阳性神经元周围形成篮状结构。结论 坐骨神经损伤后DRG内中、大型神经元的交感神经芽生及篮状结构与其Nav1.7、Nav1.8同步增加有关。这一现象可能是神经病理性疼痛异常形成的因素之一。
Objective To investigate the relationship between Nav1.7, Nav1.8 and sympathetic sprouting in dorsal root ganglion(DRG) during neuropathic pain induced by spared nerve injury(SNI).Methods The experimental rats were randomly divided into Control group(n=18) and SNI group(n=18). Mechanical withdrawal threshold(MWT) was measured on the 3rd, 7th, 14th and 21st days after surgery. The mRNA expressions and protein contents of Nav1.7, Nav1.8, growth-associated protein 43(GAP43) and tyrosine hydroxylase(TH) were detected respectively from operative side lumbar DRGs which were extracted on the 7th and 21st days, and immunofluorescence staining were performed concurrently. Results Compared with Control group,MWT in SNI group was reduced timedependently. On the 7th day and 21st day in the SNI group, mRNA expressions and protein contents of Nav1.7 and Nav1.8 in the DRGs increased synchronously with the mRNA expand protein contents of GAP43 and TH. Immunofluorescence staining showed that large-diameter neurons which expressed Nav1.7, Nav1.8 and GAP43 abnormally increased on the postoperative 21st day. Especially, the positive neurons of Nav1.7 and Nav1.8 respectively were surrounded by TH positive fibers which formed basketlike structures. Conclusion Sympathetic sprouting and basket structure on the medium and large neurons in DRG after sciatic nerve injury were related to the synchronous increasing of Nav1.7 and Nav1.8. This phenomenon may be one of the formation factors of neuropathic pain.
作者
李笑笑
陈涵
李妍妍
朱玉静
张谭
唐俊
LI Xiao-xiao;CHEN Han;LI Yan-yan;ZHU Yu-jing;ZHANG Tan;TANG Jun(Department of Anesthesiology,Shanghai Fifth People's Hospital,Fudan University,Shanghai 200240,China)
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2023年第1期57-63,共7页
Fudan University Journal of Medical Sciences
基金
复旦大学附属上海市第五人民医院院内科研助推项目(2019WYZT06)。
