摘要
目的观察芳樟醇通过瞬时受体电位香草酸1(TRPV1)通路对中枢及外周神经的影响,以及其缓解肠易激综合征(IBS)内脏高敏感的作用。方法选择36只无特定病原体动物级雌性Sprague-Dawley(SD)新生幼鼠,其中30只用于行为学实验,6只用于电生理实验。行为学实验:将30只SD新生幼鼠随机分为正常对照组(结直肠内注射0.2 mL 0.9%氯化钠溶液),新生期结肠炎性刺激(NCI)组(结直肠内注射0.2 mL 0.5%的乙酸溶液),以及芳樟醇20、50、100 mg/kg组(NCI造模成功后,在5周龄时分别予以芳樟醇20、50、100 mg/kg灌胃);在6周龄时,通过结肠扩张试验评估各组大鼠的腹部撤回反射(AWR)评分和痛阈压力值(AWR评分为3分时的充气压力值);在行为学观察结束后1 h,采用蛋白质免疫印迹法和免疫组织化学法检测各组大鼠结直肠黏膜和脊髓中的TRPV1蛋白质表达水平。电生理实验:采用6~7周龄雌性SD大鼠制作离体脊髓横切片,每只大鼠随机选取5~8个神经元进行全细胞膜片钳记录,分别记录芳樟醇、河豚毒素和抗辣椒素对胶状质区神经元自发性兴奋性突触后电流(sEPSC)的作用。统计学方法采用独立样本t检验和配对t检验。结果NCI组大鼠在40 mmHg(1 mmHg=0.133 kPa)压力下的AWR评分高于正常对照组、芳樟醇20 mg/kg组、芳樟醇50 mg/kg组、芳樟醇100 mg/kg组[(2.5±0.2)分比(1.0±0.3)、(1.5±0.3)、(1.5±0.2)、(1.5±0.2)分],在60 mmHg压力下的AWR评分高于正常对照组、芳樟醇50 mg/kg组、芳樟醇100 mg/kg组[(3.8±0.2)分比(2.3±0.4)、(2.3±0.5)、(2.0±0.3)分],差异均有统计学意义(t=4.39、2.45、3.16、3.16、3.31、2.88、5.97;P=0.001、0.034、0.010、0.010、0.008、0.028,<0.001)。NCI组大鼠的痛阈低于正常对照组、芳樟醇20 mg/kg组、芳樟醇50 mg/kg组、芳樟醇100 mg/kg组[(35.8±2.0)mmHg比(55.8±1.5)、(49.2±2.4)、(53.3±2.1)、(55.0±1.8)mmHg,差异均有统计学意义(t=-7.91、-4.28、-6.01、-7.06,P<0.001、=0.002、<
Objective To observe the effects of linalool on central and alleviating peripheral nervous system through transient receptor potential vanilloid 1(TRPV1)pathway and its role in alleviating visceral hypersensitivity in irritable bowel syndrome(IBS).Methods Thirty-six female newborn Sprague-Dawley(SD)rat pups in specific pothogen free was selected,among which 30 rats were used for behavioral experiments and 6 rats for electrophysiological experiment.In behavioral experiments,30 newborn SD rats were randomly divided into normal control group(0.2 mL 0.9%sodium chloride solution was injected into the colorectal),neonatal colonic inflammation(NCI)group(0.2 mL 0.5%acetic acid was injected into the colorectal),linalool 20,50 and 100 mg/kg groups(after NCI model successfully established,the rat were gavaged with linalool 20,50 or 100 mg/kg at 5 weeks old).At 6 weeks old,abdominal withdrawal reflex(AWR)and pressure value of pain threshold(inflation pressure value at AWR score of 3)was observed by colorectal distention test.One hour after behavioral experiments,the expression of TRPV1 at protein level in colonic mucosa and spinal cord of each group was detected by Western blotting and immunohistochemistry.In electrophysiological experiment,female SD rats aged 6 to 7 weeks were selected to make in vitro transverse sections of the spinal cord.Five to eight neurons were randomly selected from each rat for whole-cell patch-clamp recording.The effects of linalool,tetrodotoxin and anti capsaicinand on the spontaneous excitatory postsynaptic current(sEPSC)of substantiagelatinosa neurons was recorded.Independent sample t test and paired t test were used for statistical analysis.Results The AWR score of rats in NCI group was higher than those in normal control group,linalool 20 mg/kg group,linalool 50 mg/kg group and linalool 100 mg/kg group at 40 mmHg(1 mmHg=0.133 kPa)pressure(2.5±0.2 vs.1.0±0.3,1.5±0.3,1.5±0.2,and 1.5±0.2,respectively),at 60 mmHg pressure the AWR score was higher than those in normal control group,linalool 50
作者
王翀
李国华
祝荫
舒徐
陈幼祥
Wang Chong;Li Guohua;Zhu Yin;Shu Xu;Chen Youxiang(Department of Gastroenterology,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China)
出处
《中华消化杂志》
CAS
CSCD
北大核心
2023年第8期545-552,共8页
Chinese Journal of Digestion
基金
江西省自然科学基金(20202BABL216008、20212BAB206025)
江西省教育厅课题(GJJ190066)。
