摘要
目的:观察艾灸对肠易激综合征(IBS)模型小鼠行为学及其结肠色氨酸代谢相关产物的影响,探讨艾灸治疗IBS的作用机制.方法:将24只小鼠随机分为正常组、模型组、艾灸组和益生菌组,每组6只.采用2,4,6-三硝基苯磺酸(TNBS)溶液灌肠建立IBS内脏痛模型.艾灸组采用温和灸双侧足三里;益生菌组采用双歧杆菌等益生菌灌胃.治疗后进行腹部撤回反射(AWR)实验、高架十字迷宫(EPM)实验和强迫游泳实验(FST);采用免疫荧光法检测结肠5-羟色氨(5-HT)和色氨酸羟化酶1(TPH1)表达量;酶联免疫吸附测定法检测结肠色氨酸(Trp)、犬尿氨酸(Kyn)和吲哚2,3加氧酶(IDO)表达.结果:与正常组相比,模型组不同压力值下AWR评分均明显升高(P<0.01),EPM实验开臂停留时间和进入开臂次数均显著下降(P<0.01;P<0.05),FST中静止不动时间显著增加(P<0.01),结肠Trp、TPH1、IDO、5-HT及Kyn表达显著增加(P<0.01).与模型组相比,艾灸组与益生菌组AWR评分不同程度下降(P<0.05或P<0.01);EPM实验进入开臂次数均增加(P<0.05);FST中静止不动时间均减少(P<0.05);结肠Trp、TPH1、IDO及5-HT表达均减少(P<0.01或P<0.05);艾灸组开臂停留时间显著增加(P<0.01);益生菌组结肠Kyn表达显著减少(P<0.01).结论:艾灸足三里可改善IBS小鼠内脏痛与痛情绪,下调结肠TPH1、IDO、Trp、5-HT及Kyn表达.
Objective To observe the effect of moxibustion on behaviors and related products of tryptophan(Trp)metabolism in the colon of mice with irritable bowel syndrome(IBS),and to explore the mechanism of moxibustion in the IBS treatment.Methods Twenty-four mice were randomly divided into a normal group,a model group,a moxibustion group,and a probiotic group,with 6 mice in each group.The visceral pain model of IBS was established by enema with 2,4,6-trinitrobenzene sulfonic acid(TNBS)solution.Mice in the moxibustion group were treated with mild moxibustion at bilateral Zusanli(ST36),and those in the probiotic group were treated with probiotics such as Bifidobacterium by gavage.Abdominal withdrawal reflex(AWR)test,elevated plus-maze(EPM)test,and forced swimming test(FST)were performed after treatment.The expression levels of 5-hydroxytryptamine(5-HT)and tryptophan hydroxylase 1(TPH1)in the colon were detected by immunofluorescence,and the expression levels of Trp,kynurenine(Kyn),and indole-2,3-oxygenase(IDO)in the colon were detected by enzyme-linked immunosorbent assay.Results Compared with the normal group,the AWR scores were increased significantly in the model group under different pressure values(P<0.01),the open-arm staying time and open-arm entries in the EPM test were decreased significantly(P<0.01,P<0.05),the motionless time in the FST was increased significantly(P<0.01),and the expression levels of colonic Trp,TPH1,IDO,5-HT,and Kyn were increased significantly(P<0.01)in the models.Compared with the model group,the AWR scores were differently decreased(P<0.05 or P<0.01),the open-arm entries in the EPM test were increased(P<0.05),the motionless times in the FST were decreased(P<0.05),and the colonic expression levels of Trp,TPH1,IDO,and 5-HT were decreased(P<0.01 or P<0.05)in the moxibustion and probiotic groups;the open-arm staying time was significantly increased in the moxibustion group(P<0.01),and the colonic expression level of Kyn was significantly decreased in the probiotic group(P<0.01).Conclusion Moxibus
作者
陈嘉宜
朱笑吉
彭洋
刘慧荣
吴焕淦
周次利
CHEN Jiayi;ZHU Xiaoji;PENG Yang;LIU Huirong;WU Huangan;ZHOU Cili(Shanghai University of Traditional Chinese Medicine,Shanghai,201203,China;Shanghai Research Institute of Acupuncture and Meridian,Shanghai,200030,China;Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai,200437,China)
基金
国家自然科学基金项目,No.81873377
国家中医药领军人才"岐黄学者"
上海市卫生健康委员会卫生行业临床研究专项项目,No.202040250
上海市自然科学基金项目,No.16ZR1433500
上海中医药大学"杏林百人"计划
上海中医药大学研究生创新培养专项,No.Y2020040.
关键词
灸法
穴
足三里
肠易激综合征
内脏痛
色氨酸代谢
焦虑
抑郁
小鼠
Moxibustion Therapy
Point,Zusanli(ST36)
Irritable Bowel Syndrome
Visceral Pain
Tryptophan Metabolism
Anxiety:Depression:Mice
