摘要
为挖掘桉树木材形成重要NAC转录因子,以尾巨桉DH32-29的转录组测序数据为基础,克隆了一个桉树NAC基因EugNAC007,并对其功能进行了初步研究。序列分析表明:EugNAC007编码序列全长1711 bp,预测编码蛋白含569个氨基酸,分子量为64.32 KD,定位于细胞核。EugNAC007蛋白在N端具有一个典型的NAC结构域。系统进化分析表明EugNAC007蛋白与拟南芥中调控细胞分裂的AtNTM1亲缘关系较近。实时荧光定量PCR结果显示EugNAC007在木质部中优势表达。在杨树中过表达该基因抑制了植株株高生长。推测EugNAC007可能通过调控细胞分裂的形式参与木材形成过程,但具体作用途径和机制待进步一研究。该研究为深入挖掘桉树木材形成相关重要NAC转录因子提供参考。
To identify key NAC transcription factors involved in wood formation,a NAC transcription factor gene EugNAC007 of the clone DH32-29 of Eucalyptus urophylla×E.grandis was cloned based on RNA sequencing data and its function analyzed.The coding DNA sequence length of the EugNAC007 gene was found to be 1711 bp and encodes an acidic protein with 569 amino acid residues of molecular weight of 64.32 KD.The EugNAC007 protein contained a typical NAC domain at the N terminus and shared relatively high homology with the Arabidopsis NAC with TRANSMEMBRANE MOTIF1(AtNTM1)that regulates cell division.The results of RT-qPCR showed that EugNAC007 was predominantly expressed in xylem tissues.Overexpression of EugNAC007 in poplar suppressed growth of tree height.These results indicated that the EugNAC007 may participate in the processes of wood formation through the regulation of cell division,but the regulation mechanism is still unclear.This study advanced deep excavation of NAC transcription factors involved in wood formation processes in Eucalyptus.
作者
何沙娥
欧阳林男
杨嘉麒
郑嘉琪
陈少雄
HE Shae;OUYANG Linnan;YANG Jiaqi;ZHENG Jiaqi;CHEN Shaoxiong(Research Institute of Fast-growing Trees,Chinese Academy of Forestry,Zhanjiang 524022,Guangdong,China)
出处
《桉树科技》
2023年第4期1-8,共8页
Eucalypt Science & Technology
基金
广东省基础与应用基础研究基金联合基金(2020A1515110931)
中国林业科学研究院基本科研业务费专项(CAFYBB2021MA010)。
关键词
桉树
NAC转录因子
基因克隆
基因功能
Eucalyptus
NAC transcription factor
gene cloning
gene function
