摘要
为了明确油菜NAC家族转录因子在非生物胁迫响应中的功能,通过油菜全基因组鉴定获得了379个BnNAC转录因子家族成员,系统进化分析将其分为17个亚族。启动子作用元件分析显示BnNAC家族成员广泛参与光响应、干旱胁迫响应、低温胁迫响应、生物钟调控等进程,同时参与ABA、茉莉酸甲酯、赤霉素、生长素、水杨酸等激素信号途径。不同胁迫条件下基因表达分析发现,BnNAC家族基因受温度、盐、渗透等胁迫及ABA诱导调控。过表达BnNAC253基因使拟南芥对盐胁迫、渗透胁迫和ABA处理更为敏感。
To clarify the function of NAC family transcription factors responding to abiotic stress in Brassica napus, 379 BnNAC transcription factors were identified by whole genome screen, and were subsequently divided into 17 subfamilies by phylogenetic analysis. Promoter cis-acting element analysis showed that BnNAC were involved in light response, drought response, cold response, meristem development, circadian control, as well as ABA, methyl jasmonate, gibberellin, auxin, salicylic acid response pathways. Gene expression level under different stress conditions showed that BnNAC family gene was regulated by high temperature, cold, salt, osmotic stress and ABA treatment. Overexpression of BnNAC253 gene in Arabidopsis decreased tolerance to salt stress, osmotic stress and ABA treatment.
作者
王勇锋
杨翠玲
冷秋丽
陈浩
陈玉平
王道杰
WANG Yong-feng;YANG Cui-ling;LENG Qiu-li;CHEN Hao;CHEN Yu-ping;WANG Dao-jie(State Key Laboratory of Crop Stress Adaptation and Improvement,School of Life Sciences,Henan University,Kaifeng 475004,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2020年第4期545-553,共9页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(31671728)。
