摘要
以马铃薯野生材料W3(耐寒材料)和Cph12(敏感材料)幼苗为试验材料,采用不同浓度脱落酸(ABA)叶面喷施,通过低温胁迫(-2℃,2 h)后,观察幼苗表观性状,并测定马铃薯叶片叶绿素、丙二醛(MDA)、脯氨酸(Pro)含量,超氧化物歧化酶(SOD)活性。结果表明,外源喷施ABA可以有效缓解由低温胁迫引起的叶绿素降解,降低植物中MDA积累量,增加体内SOD活性和Pro含量。抗寒性与生理指标相关性分析结果表明:添加外源ABA后,马铃薯野生材料W3的抗寒性与叶绿素、MDA、Pro含量,SOD活性变化呈显著相关,抗寒性进一步增强,霜冻敏感野生材料Cph12的抗寒性无显著变化。因此,叶面喷施外源ABA能通过调控马铃薯的渗透调节和抗氧化能力来减轻细胞膜的损伤,有效缓解低温胁迫带来的伤害;同时外源ABA能增强W3(耐寒材料)的耐寒性。
We used wild potato material W3(cold resistant material)and Cph12(sensitive)seedlings as experimen⁃tal materials.Different concentrations of abscisic acid(ABA)were used to spray on the leaf,then after low tempera⁃ture stress(-2℃,2 h),we observed the apparent properties of the seedlings and determined the change of physiologi⁃cal indicators such as malondialdehyde(MDA),superoxide dismutase(SOD),proline(Pro)content.The results showed that ABA spraying could effectively alleviate the chlorophyll degradation caused by low temperature stress,reduced the accumulation of MDA in plants,and increased the content of SOD and Pro.The correlation analysis be⁃tween cold resistance and physiological indexes showed that potato material W3 was significantly correlated with the changes of chlorophyll,MDA,SOD and Pro content after ABA treatment.The cold resistance was further enhanced,while the cold resistance of frost-sensitive wild material Cph12 showed no significant changes.Therefore,ABA spraying on the leaf can reduce the damage of cell membrane by regulating the osmotic regulation and antioxidant capacity of potato,and effectively alleviate the damage caused by low temperature stress.ABA could also enhance the cold resistance of W3.
作者
吴巧玉
何天久
WU Qiaoyu;HE Tianjiu(Institute of Biological Technology,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)
出处
《东北农业科学》
2023年第5期88-91,共4页
Journal of Northeast Agricultural Sciences
基金
贵州省科学技术基金项目(黔科合基础【2018】1152)。
关键词
马铃薯
脱落酸
低温胁迫
生理特性
Potato
Abscisic acid
Low temperature stress
Physiological characteristics
