摘要
目的探讨中等有氧运动和高强度间歇运动对非酒精性脂肪肝小鼠心肌线粒体自噬的影响及可能机制。方法将40只3周龄雄性C57BL/6J小鼠随机分为普通饲养组(Chow组,n=10)和高脂饲养组(HFD组,n=30)。第18周,将HFD组体重超过普通饮食组20%~30%(包含20%)者判定为肥胖小鼠(n=26),通过随机抽选2只小鼠做肝油红O染色,确定NAFLD小鼠模型建立成功。随机筛选出16只NAFLD小鼠分成中等强度有氧运动组(MICT组)和高强度间歇有氧运动组(HIIT组),每组8只,分别对两组进行8周的运动训练,干预结束后称重取材。Masson染色观察心肌纤维化情况;透射电镜观察心肌细胞超微结构;Western blot检测心肌中线粒体自噬和线粒体生物发生等相关蛋白表达。结果(1)与Control组相比,Model组体重显著增加、心指数明显下降;与Model组相比,MICT组和HIIT组体重明显增加、MICT组心指数上升。(2)Masson结果显示,与Control组相比,Model组心肌内胶原纤维含量显著增加,且电镜下心肌肌纤维排列杂乱、断裂,心肌形态杂乱不堪,线粒体肿胀、嵴发生断裂且模糊不清并夹杂多个脂滴;与Model组相比,MICT组和HIIT组心肌内胶原纤维含量显著性减少,透射电镜下心肌肌纤维排列略有恢复,z线清晰可见,线粒体变性程度略有改善,且脂滴也略有减少,其中,MICT组对心脏组织结构改善效果优于HIIT组。(3)Western blot结果显示,与Control组相比,Model组心肌组织中PINK1和Beclin1表达水平无明显变化,Parkin、LAMP1和PGC-1α表达显著下降(P<0.01),蛋白表达和LC3-Ⅱ/LC3-Ⅰ比值显著升高(P<0.05,P<0.01);与Model组相比,MICT组和HIIT组PINK1和Beclin1表达水平无明显变化,PGC-1α表达呈上调趋势,其中MICT组Parkin、LAMP1表达显著升高(P<0.05),p62蛋白表达和LC3-Ⅱ/LC3-Ⅰ比值明显降低(P<0.05,P<0.01),HIIT组Parkin、LAMP1表达水平具有上调趋势,LC3-Ⅱ/LC3-Ⅰ比值和p62蛋白表达明显降低(P<0.05,P<0.01)。结论不�
ObjectiveTo investigate the effects of moderate aerobic exercise and high intensity intermittent exercise on myocardial mitophagy in mice with nonalcoholic fatty liver disease and its possible mechanism.MethodsForty 3-week-old male C57BL/6J mice were randomly divided into a normal feeding group(Chow group,n=10)and high fat-fed group(HFD group,n=30).At week 18,mice in the HFD group with a body weight exceeding 20%~30%of the ordinary diet group were considered obese mice(n=26).Two mice were randomly selected for liver oil red O staining to confirm successful establishment of the NAFLD mouse model.Sixteen NAFLD mice were randomly selected and divided into a moderate intensity aerobic exercise group(MICT group)and high intensity intermittent aerobic exercise group(HIIT group)with eight mice in each group.The two groups were subjected to exercise training for 8 weeks,and samples were weighed after treatment.Masson staining was used to observe myocardial fibrosis.The ultrastructure of myocardial cells was observed by transmission electron microscopy.Mitophagy and mitochondrial biogenesis were assessed by Western blot.ResultsCompared with the Control group,the body weight and heart index in the Model group were significantly increased and decreased,respectively.Compared with the Model group,the body weight of MICT and HIIT groups was increased significantly,and the heart index of the MICT group was increased.Masson staining showed that,compared with the Control group,collagen fiber content in the myocardium in the Model group was significantly increased,and the myocardial fibers were disorganized and broken in electron microscopy.The myocardial morphology was disorganized,mitochondria were swollen,cristae were broken and blurred,and lipid droplets were observed.Compared with the Model group,the myocardial collagen fiber content in MICT and HIIT groups was significantly reduced,the myocardial fiber arrangement had slightly recovered in transmission electron microscopy,the z-line was clearly visible,the degree of mitochon
作者
何苗
李佳航
刘鑫
杨威
吴良文
范晶晶
HE Miao;LI Jiahang;LIU Xin;YANG Wei;WU Liangwen;FAN Jingjing(Wuhan University of Physical Education,College of Sports Medicine,Wuhan 430079;China.2.Wuhan University of Physical Education,Hubei Key Laboratory of Sports Training Monitoring,Wuhan 430079)
出处
《中国比较医学杂志》
CAS
北大核心
2023年第11期1-9,共9页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金(81701391)
湖北省自然科学基金计划项目(2023AFB700)
武汉体育学院中青年科研团队项目(21KT08)。
关键词
中等连续有氧运动
高强度间歇运动
NAFLD心肌病
线粒体自噬
moderate continuous aerobic exercise
high-intensity intermittent exercise
NAFLD cardiomyopathy
mitophagy
