摘要
目的 探究IgA肾病患者血清微小核糖核酸(micro RNA,miR)-346及分泌型卷曲相关蛋白4(secreted frizzled-related protein 4, SFRP-4)水平表达与病情严重程度及预后的关系。方法 选取2018年3月~2020年1月在成都市第六人民医院接受治疗的102例IgA肾病患者为研究对象,根据肾间质纤维化(renal interstitial fibrosis,RIF)程度将IgA肾病患者分为轻度组(n=35)、中度组(n=43)和重度组(n=24),根据随访结果将IgA肾病患者分为预后良好组(n=70)和预后不良组(n=32),另选取同期健康体检者50例为对照组。比较患者一般资料及血清miR-346和SFRP-4水平;Spearman相关性分析IgA肾病患者血清miR-346和SFRP-4水平与病情严重程度之间的关系;Logistic回归分析IgA肾病患者预后不良的影响因素。结果 随着IgA肾病患者病情程度的增加,血清肌酐(serum creatinine,SCr)、血尿素氮(blood urea nitrogen,BUN)、24 h尿蛋白定量、尿酸(uric acid,UA)和SFRP-4水平逐渐升高,差异均有统计学意义(F=183.466,66.795,46.281,88.920,147.171,均P <0.05);血红蛋白(hemoglobin,Hb)、清蛋白(albumin,Alb)、肾小球滤过率(estimated glomerular filtration rate,eGFR)和miR-346水平逐渐降低,差异均有统计学意义(F=15.919,55.071,132.759,96.735,均P <0.05);Spearman相关性分析结果显示,IgA肾病患者血清miR-346与病情程度呈显著负相关(r=-0.673,P <0.05),血清SFRP-4与病情程度呈显著正相关(r=0.532,P<0.05);预后不良组患者SCr(164.39±29.21 mmol/L),BUN(12.29±2.79 mmol/L),24 h尿蛋白定量(2.80±0.79g/24h),UA(398.41±52.63μmol/L),SFRP-4(2.90±0.41 ng/ml)水平均高于预后良好组(121.68±26.89 mmol/L,8.85±2.47 mmol/L,2.26±0.65 g/24h,367.36±43.61μmol/L,2.18±0.35 ng/ml),差异具有统计学意义(t=7.184,6.264,3.836,3.123,9.128,均P <0.05);Hb(119.56±22.25 g/ml),Alb(28.01±4.17 g/ml),e GFR(53.53±10.14 ml/min/1.73m2),miR-346(0.43±0.10 vs)水平均低于预后良好组(132.48±24.15 g/ml,34.22±5.82 g/ml,70.41±14.21ml/min/1.73m2,0.71±0.1
Objective To investigate the relationship between serum micro RNA(miR)-346 and secreted frizzled related protein 4(SFRP-4)levels and severity and prognosis in patients with IgA nephropathy.Methods 102 patients with IgA nephropathy who received treatment at the Sixth People’s Hospital of Chengdu from March 2018 to January 2020 were selected as the research subjects,according to the degree of renal interstitialfibrosis(RIF),patients with IgA nephropathy were grouped into mild group(n=35),moderate group(n=43)and severe group(n=24),according to the follow-up results,patients with IgA nephropathy were grouped into a good prognosis group(n=70)and a poor prognosis group(n=32),and 50 healthy subjects during the same period were selected as control group.General information and serum levels of miR-346 and SFRP-4 were compared.Spearman correlation analysis was applied to analyze the relationship between the serum levels of miR-346 and SFRP-4 and the severity of IgA nephropathy and Logistic regression analysis was applied to analyze the influencing factors of poor prognosis in patients with IgA nephropathy.Results As the severity of IgA nephropathy increased,the levels of serum creatinine(SCr),blood urea nitrogen(BUN),24-hour urinary protein quantitation,uric acid(UA),and SFRP-4 gradually increased,and the differences were statistically significant(F=183.466,66.795,46.281,88.920,147.171,all P<0.05).The levels of hemoglobin(Hb),albumin(Alb),estimated glomerularfiltration rate(eGFR),and miR-346 gradually decreased,and the differences were statistically significant(F=15.919,55.071,132.759,96.735,all P<0.05).Spearman correlation analysis showed that there was a obvious negative correlation between serum miR-346 and severity of IgA nephropathy(r=-0.673,P<0.05),and there was a obvious positive correlation between serum SFRP-4 level and severity of disease(r=0.532,P<0.05).The levels of SCr(164.39±29.21 mmol/L),BUN(12.29±2.79 mmol/L),24-hour urinary protein quantitation(2.80±0.79 g/24h),UA(398.41±52.63µmol/L),and SFRP-4(
作者
赵玲
金巧玲
黄俊
高兴
宿林
ZHAO Ling;JIN Qiaoling;HUANG Jun;GAO Xing;SU Lin(Department of Nephrology,the Sixth of People’s Hospital of Chengdu City,Chengdu 610051,China;Ward of Drug Clinical Trial PhaseⅠ,the Sixth of People’s Hospital of Chengdu City,Chengdu 610051,China;Department of Nephrology,West China Fourth Hospital,Sichuan University,Chengdu 610041,China)
出处
《现代检验医学杂志》
CAS
2023年第6期30-34,41,共6页
Journal of Modern Laboratory Medicine
