摘要
为了揭示无芒雀麦(Bromus inermis L.)在盐胁迫条件下的生理响应规律,探索了盐胁迫条件下乌苏1号无芒雀麦渗透调节物质和激素变化情况。通过测定NaCl(200 mmol/L)胁迫下无芒雀麦根、茎和叶中可溶性糖(SS)、可溶性蛋白(SP)、脯氨酸(Pro)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、脱落酸(ABA)、生长素(IAA)和玉米素核苷(ZR)含量变化,分析在盐胁迫条件下其根、茎和叶中的主要响应物质。结果显示,无芒雀麦根、茎和叶的可溶性糖、可溶性蛋白、脯氨酸含量均随盐胁迫时间的延长而增加,3种渗透调节物质根中含量低于茎和叶;盐胁迫下无芒雀麦根、茎和叶的超氧化物歧化酶和过氧化氢酶活性先降低后升高,其中叶片超氧化物歧化酶活性显著高于茎和根,无芒雀麦各部位过氧化物酶活性显著增加,根部过氧化物酶活性高于叶片,表明超氧化物歧化酶响应主要在茎叶,过氧化物酶主要在根部响应;盐胁迫后无芒雀麦根脱落酸含量先升高后降低,表明脱落酸胁迫响应的时间主要在前期,24 h达到峰值;茎和叶脱落酸含量持续增加,且茎和叶片中脱落酸含量高于根部;盐胁迫对无芒雀麦各部位生长素含量影响较大,根部生长素含量低于茎和叶;盐胁迫下无芒雀麦根玉米素核苷含量先升高后降低,叶片玉米素核苷含量持续升高,且叶片中玉米素核苷含量高于茎和根,说明玉米素核苷主要在叶片中响应。总体而言,无芒雀麦在受到盐胁迫时,主要通过茎和叶增加可溶性糖、可溶性蛋白、脯氨酸含量来维持细胞的渗透平衡,超氧化物歧化酶、过氧化氢酶、过氧化物酶活性增加,减少了活性氧的影响。盐胁迫下,生长素和玉米素核苷、脱落酸均有响应,脱落酸是无芒雀麦响应盐胁迫的主要激素。
In order to uncover the physiological reaction of Bromus inermis Leyss.cv.Wusu No.1 in saline-alkali land,the alterations of osmotic adjustment materials and hormones under salt stress were investigated.In this study,Bromus inermis was taken as the research object,and the content changes of soluble sugar(SS),soluble protein(SP),proline(Pro),superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),abscisic acid(ABA),auxin(IAA),and zeatin nucleoside(ZR)in the roots,stems,and leaves of smooth brome under NaCl(200 mmol/L)stress were measured.The results showed that:The content of soluble sugar,soluble protein,and proline in the roots,stems,and leaves of smooth bromegrass increased with the prolongation of salt stress time,and the content of three osmotic regulating substances in the roots was lower than that in the stems and leaves;Under salt stress,the superoxide dismutase and catalase activities of roots,stems,and leaves of smooth bromegrass first decreased and then increased.The superoxide dismutase activity of leaves was significantly higher than that of stems and roots,and the peroxidase activity of various parts of smooth bromegrass was significantly increased.The peroxidase activity of roots was higher than that of leaves,indicating that superoxide dismutase response was mainly in stems and leaves,and peroxidase response was mainly in roots;After salt stress,the abscisic acid content in the roots of smooth bromegrass increased first and then decreased,indicating that the response time to abscisic acid stress was mainly in the early stage,reaching its peak at 24 hours;The abscisic acid content in stems and leaves continued to increase,and the abscisic acid content in stems and leaves was higher than that in roots;Salt stress had a significant impact on the auxin content in various parts of smooth bromegrass,with the auxin content in the roots being lower than that in the stems and leaves;Under salt stress,the zeatin nucleoside content in the roots of smooth bromegrass first increased and then decreased,while the
作者
孙雪
朝木力嘎
孙强
周乐
孙宇阳
张延辉
SUN Xue;Chaomuliga;SUN Qiang;ZHOU Le;SUN Yu-yang;ZHANG Yan-hui(Key Laboratory of Grassland Resources and Ecology of Western Arid Desert Area,Ministry of Education,Urumqi 830052,China;Xinjiang Key Laboratory of Grassland Resources and Ecology,Urumqi 830052,China;College of Grassland Science,Xinjiang Agricultural University,Urumqi 830052,China;Grassland Master Station of Xinjiang Uygur Autonomous Region,Urumqi 830049,China;Grassland Station of Kashgar,Xinjiang,Kashgar 844599,China)
出处
《新疆农业大学学报》
CAS
2023年第3期180-186,共7页
Journal of Xinjiang Agricultural University
基金
新疆维吾尔自治区自然科学基金项目(2020D01A62)。
关键词
无芒雀麦
NACL胁迫
渗透调节物质
抗氧化酶
内源激素
Bromus inermis
NaCl stress
osmoregulation substance
antioxidant enzyme
endogenous hormones
