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长途运输影响安格斯育肥牛肝脏功能关键基因的筛选

Screening of Key Genes Affecting Liver Function in Angus Fattening Cattle After Long-distance Transportation
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摘要 【目的】研究长途运输对育肥牛体重、屠宰率及肝脏mRNA表达谱的影响,筛选长途运输影响肝脏功能的关键基因。【方法】选用体重相近的安格斯育肥公牛22头,随机分为2组,对照组9头就地屠宰,试验组13头经长途运输后屠宰,观察长途运输对体重和屠宰率的影响;此外,两组分别采集3头体重相近牛的肝脏组织,进行转录组测序(RNA-Seq),筛选差异表达基因,并对其进行GO功能和KEGG通路富集分析。【结果】长途运输后牛活体重降低4.5%,屠宰率较对照组无显著差异(P>0.05);RNA-Seq结果发现,长途运输组肝脏组织有182个基因显著上调表达,91个基因显著下调表达。GO功能与KEGG通路富集分析显示,182个上调表达基因显著富集在刺激反应的调节、过氧化物酶体和氧化还原酶活性,以及cAMP、MAPK和长寿调控通路等,与能量代谢及肝细胞损伤密切相关。根据GO功能与KEGG通路富集分析结果,筛选出4个与育肥牛长途运输后肝脏能量代谢变化、肝脏细胞损伤及损伤后适应性保护机制密切相关的基因:ACOX 3、FOS、HSPA 1 A、HSPA 6。【结论】安格斯育肥牛经长途运输后,活体重较运输前降低4.5%,筛选得到4个长途运输影响安格斯育肥牛肝脏功能的关键基因:ACOX 3、FOS、HSPA 1 A与HSPA 6。研究结果为今后肉牛运输应激发生机理、预防和治疗研究提供参考。 【Objective】This experiment was conducted to study the effects of long-distance transportation on body weight,slaughter rate and liver mRNA expression profile of fattening cattle and to screen for key genes affecting liver function in long-distance transportation.【Method】Twenty-two Angus fattening bulls of similar weight were randomly divided into 2 groups,9 of which were slaughtered on the spot in the control group,and 13 of which were slaughtered after long-distance transportation in the experimental group,and the effect of long-distance transportation on body weight and slaughter rate was observed.In addition,three bovine liver tissues of similar weight were collected in the two groups.Transcriptome sequencing(RNA-Seq)was performed to screen differentially expressed genes,and GO functional annotation and KEGG pathway enrichment analysis were performed on them.【Result】The live body weight of cattle decreased by 4.5%after long-distance transportation,and there was no significant difference in slaughter rate compared with the control group(P>0.05).The RNA-Seq results showed that 182 genes were significantly upregulated and 91 genes were significantly downregulated in liver tissues of the long-distance transportation group.GO enrichment and KEGG pathway analysis showed that 182 up-regulated genes were significantly enriched in the terms of regulation of response to stimulus,peroxisome,oxidoreductase activity,cAMP signaling pathway,MAPK signaling pathway,and longevity regulating pathway.According to the results of GO enrichment and KEGG pathway analysis,four genes(ACOX 3,FOS,HSPA 1 A,HSPA 6)were screened out that were closely related to the changes of liver energy metabolism,liver cell damage and post-injury adaptive protection mechanism of fattening cattle after long-distance transportation.【Conclusion】The live weight of Angus fattening cattle was reduced by 4.5%after long-distance transportation.Four key genes ACOX 3,FOS,HSPA 1 A and HSPA 6 affecting liver function of Angus fattening cattle after l
作者 孙文阳 谢建亮 施安 陈志龙 姜武 王瑞刚 侯鹏霞 梁小军 SUN Wenyang;XIE Jianliang;SHI An;CHEN Zhilong;JIANG Wu;WANG Ruigang;HOU Pengxia;LIANG Xiaojun(Institute of Animal Science,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,China;Guyuan Animal Husbandry and Fisheries Technology Extension and Service Center,Guyuan 756000,China;Guyuan Branch of Ningxia Academy of Agriculture and Forestry Sciences,Guyuan 756000,China;Ningxia Benwang Ecological Agriculture Co.,Ltd.,Yongning 750100,China)
出处 《中国畜牧兽医》 CAS CSCD 北大核心 2023年第10期4096-4105,共10页 China Animal Husbandry & Veterinary Medicine
基金 宁夏回族自治区农业科技自主创新项目(NGSB-2021-12-02) 财政部和农业农村部——国家现代农业产业技术体系专项(CARS-37) 国家重点研发计划(2021YFD1100508、2022YFD1602501)。
关键词 长途运输 屠宰率 肝脏功能 肉牛 long-distance transportation slaughter rate liver function beef cattle
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