摘要
目的:探索人工培殖冬虫夏草Cordyceps sinensis(BerK.)Sacc.外观品质形成的分子基础。方法:以重庆和康定人工培殖冬虫夏草僵虫为材料,利用Illumina Hiseq 2500高通量测序技术进行转录组测序分析,基于差异倍数(FC)和错误发现率(FDR)筛选差异表达基因,具体筛选条件为|log2FC|>1、FDR<0.05。结果:共获得21942个转录本,利用DEGSeq筛选获得1575个差异表达基因,其中上调表达基因626个、下调表达基因949个;基因本体(GO)数据库和京都基因与基因组百科全书(KEGG)富集分析结果显示,这些差异表达基因主要集中于氨基酸代谢、折叠-分选-降解、糖代谢、转运和分解代谢、辅酶因子和微生物代谢等途径;筛选到66个颜色变化差异表达基因,包含46个氧化还原酶活性相关基因、12个过氧化物酶体相关基因、3个单加氧酶活性调节相关基因、3个黑化反应相关基因、2个活性氧代谢相关基因,其中tyr1、TYR基因可能是冬虫夏草僵虫颜色变化的关键功能基因。结论:利用高通量测序技术和生物信息分析获得了人工培殖冬虫夏草僵虫转录组信息特征,为阐明人工培殖冬虫夏草僵虫颜色变化的调控机制研究提供参考。
Objective:To explore the molecular mechanism of appearance quality formation in artificially cultivated Cordyceps sinensis(BerK.)Sacc.Methods:This study performed transcriptome sequencing using Illumina Hiseq 2500 high-throughput sequencing technology on the Chongqing artificially cultivated C.sinensis sclerotium before stroma development(CQ)and Kangding artificially cultivated C.sinensis sclerotium before stroma development(KD),and differentially expressed genes(DEGs)were obtained based on the screening conditions of|log2FC|>1 and FDR<0.05.Results:A total of 21942 transcripts were obtained,and 1575 DEGs were screened by DEGSeq.There were 626 up-regulated genes and 949 down-regulated genes,Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis showed that these DEGs were mainly involved in amino acid metabolism,folding-sorting-degradation,glycometabolism,transport,catabolism,co-enzyme factor and microbial metabolism,and other pathways.66 DEGs were screened,including 46 enzyme genes involved in oxidoreductase activity,12 genes involved in the peroxisome,three genes involved in monooxygenase activity,three genes involved in melanization reactions,and two genes involved in reactive oxygen metabolism,among which tyr1 and TYR may be the key functional gene for the color formation of C.sinensis.Conclusion:High-throughput sequencing technology and bioinformatics analysis were used to obtain the transcriptomic information characteristics of artificially cultivated C.sinensis sclerotium before stroma development,which laid a foundation for clarifying the regulation mechanism of color formation of artificially cultivated C.sinensis sclerotium before stroma development.
作者
邓小书
卫秋阳
谭发银
郭连安
王嘉
邢康康
陈仕江
DENG Xiao-shu;WEI Qiu-yang;TAN Fa-yin;GUO Lian-an;WANG Jia;XING Kang-kang;CHEN Shi-jiang(Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China;Chongqing College of Traditional Chinese Medicine,Chongqing 402760,China)
出处
《中国现代中药》
CAS
2023年第8期1635-1645,共11页
Modern Chinese Medicine
基金
重庆市自然科学基金面上项目(CSTB2022NSCQ-MSX0874,cstc2021jcyj-msxmX0762)
重庆市中药研究院自主科技计划项目(zzkjjh2022006)
重庆市基本科研业务费项目(cstc2020jxjl-jbky00004)
重庆市技术创新与应用发展专项(cstc2020jscx-msxmX0102)
重庆市中药研究院科研发展基金项目(ygfz20220008)。
关键词
冬虫夏草
僵虫
高通量测序
转录组
Cordyceps sinensis(BerK.)Sacc.
C.sinensis sclerotium before stroma development
high-throughput sequencing
transcriptome
