摘要
本研究旨在探讨运动对高脂喂养诱导的肥胖小鼠前额叶PGC-1α、鸢尾素(irisin)、BDNF、氧化应激、炎症及认知功能的影响,为改善肥胖群体认知障碍的运动康复与靶标筛选提供实验依据。3月龄清洁级雄性C57BL/6J野生型小鼠,随机分为对照组、高脂饮食组、高脂饮食+中等强度持续运动组、高脂饮食+中高强度间歇运动组,每组10只。高脂饮食+中等强度持续运动组和高脂饮食+中高强度间歇运动组小鼠在高脂喂养12周,接受8周中等强度持续运动或中高强度间歇运动。行为学结果显示,与对照组比较,高脂饮食组小鼠脱粘实验反应时长显著增加(P<0.01),Y迷宫试验自发交替百分比显著降低(P<0.01),新型物体识别探索时间百分比显著降低(P<0.01),结果表明,高脂饮食可导致小鼠认知功能障碍。Nissl染色和Western印迹结果显示,与对照组比较,高脂饮食组小鼠前额叶神经元尼氏体溶解和细胞凋亡增加(P<0.01),前额叶PGC-1α、Irisin、BDNF、IL-10和T-SOD水平显著降低(P<0.05,P<0.01),IL-1β、TNF-α、NF-κB、裂解胱天蛋白酶3(cleaved caspase-3)、Bax/Bcl-2、ROS和MDA水平显著升高(P<0.01),表明高脂饮食导致小鼠前额叶炎症、氧化应激和细胞凋亡,引发前额叶损伤。与高脂饮食组比较,高脂饮食+中等强度持续运动组和高脂饮食+中高强度间歇运动组,小鼠脱粘实验反应时长缩短(P<0.01),Y迷宫探索自发交替百分比显著增加(P<0.05,P<0.01),新型物体识别探索时间百分比显著增加(P<0.05,P<0.01),表明持续有氧和间歇运动均可显著改善肥胖小鼠认知功能;前额叶神经元尼氏体溶解和细胞凋亡减少(P<0.05,P<0.01),PGC-1α、Irisin、BDNF、IL-10和T-SOD水平显著升高(P<0.01),IL-1β、TNF-α、NF-κB、裂解胱天蛋白酶3、Bax/Bcl-2、ROS和MDA水平显著降低(P<0.01),表明持续有氧和间歇运动均可降低前额叶炎症、氧化应激和细胞凋亡,改善肥胖诱导的前额叶损伤
This study aimed to investigate the effects of exercise on prefrontal PGC-1α,Irisin,BDNF,oxidative stress,inflammation,and cognitive function in high-fat diet-induced obese mice,which may provide experimental evidence of exercise rehabilitation methods and target screening for obesity.Three-month-old male C57BL/6J wild-type mice were randomly divided into four groups:control,high-fat diet,high-fat diet with moderate intensity continuous training,and high-fat diet with high-intensity interval training group,with 10 mice in each group.The mice in the high-fat diet with moderate intensity continu-ous training group and the high-fat diet with high-intensity interval training group received 8 weeks of moderate-intensity continuous training or high-intensity interval training after 12-week high-fat feeding.Behavioral results showed that compared with the control group,reaction time of adhesive removal test was significantly increased(P<0.01),and spontaneous alternation rate in Y-maze test and exploration time in the novel object recognition test were significantly decreased(P<0.01)in the high-fat diet group,indicating that high-fat diet led to cognitive dysfunction in mice.Results showed that compared with the control group,Nissl bodies dissolution and apoptosis were significantly increased(P<0.01),levels of PGC-1α,Irisin,BDNF,IL-10,and T-SOD were significantly deceased(P<0.05,P<0.01),levels of IL-1β,TNF-α,NF-κB,cleaved Caspase-3,Bax/Bcl-2,ROS,and MDA were significantly in-creased in the prefrontal lobe(P<0.01),indicating that high-fat diet induced excessive inflammation,oxidative stress,and apoptosis,which were conducive to prefrontal lobe damage.Compared with the high-fat diet group,moderate intensity continuous or high-intensity interval training decresed reaction time of adhesive removal test(P<0.01),increased spontaneous alternation rate of Y-maze test and ex-ploration time of novel object recognition test(P<0.05,P<0.01),indicating that both continuous and interval training improved cognitive function in obes
作者
张鹏鹏
ZHANG Peng-Peng(Department of Physical Education,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China)
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2023年第9期1346-1355,共10页
Chinese Journal of Biochemistry and Molecular Biology
基金
山西省教育科学“十四五”规划2021年度课题(No.SZ-21101)资助。
关键词
鸢尾素
脑源性神经营养因子
运动
前额叶
认知功能
炎症
氧化应激
irisin
brain-derived neurotrophic factor(BDNF)
exercise
prefrontal
cognitive function
inflammation
oxidative stress
