摘要
目的探讨莫西沙星(Moxi)下调长链非编码RNA生长抑制特异性基因(lncRNA GAS5)通过非受体型蛋白酪氨酸激酶(JAK)/信号传导及转录激活蛋白(STAT)信号通路对肺炎支原体肺炎的保护作用。方法将BALB/c小鼠随机分为对照组、模型组、实验组和联合组。模型组、实验组和联合组小鼠用鼻滴肺炎支原体菌液的方法建立肺炎支原体肺炎模型。实验组和联合组腹腔注射100 mg·kg^(-1)莫西沙星治疗;在此基础上,联合组尾静脉注射过表达质粒pc-GAS5,对照组和模型组均腹腔注射等量的0.9%NaCl。用酶联免疫吸附试验法检测小鼠血清中白细胞介素-1β(IL-1β)和IL-6水平,用生化法检测小鼠血清中超氧化物歧化酶(SOD)水平,用实时荧光定量聚合酶链反应法检测小鼠肺组织中lncRNA GAS5的表达水平,用蛋白质印迹法检测小鼠肺组织中B淋巴细胞瘤-2基因(Bcl-2)、JAK2和STAT3的表达水平。结果对照组、模型组和实验组血清中IL-1β含量分别为(64.03±14.19)、(254.85±63.79)和(157.46±42.93)pg·mL^(-1),IL-6含量分别为(22.61±5.01)、(184.14±39.50)和(132.64±25.47)pg·mL^(-1),SOD活性分别为(50.38±11.75)、(28.17±6.71)和(37.67±8.09)U·mL^(-1),肺组织中lncRNA GAS5相对表达水平分别为1.00±0.16、3.78±0.69和2.41±0.54;对照组、模型组、实验组和联合组肺组织中Bcl-2蛋白相对表达水平分别为1.00±0.17、0.65±0.14、0.82±0.16和0.62±0.13,磷酸化JAK2/JAK2比值分别为1.00±0.20、4.98±1.01、2.13±0.52和4.21±0.92,磷酸化STAT3/STAT3比值分别为1.00±0.18、3.77±0.78、2.65±0.64和3.49±0.75。模型组的上述指标与对照组相比,在统计学上差异均有统计学意义(P<0.05,P<0.001);实验组的上述指标与模型组相比,在统计学上差异均有统计学意义(P<0.05,P<0.01,P<0.001);联合组的上述指标与实验组相比,在统计学上差异均有统计学意义(P<0.05,P<0.001)。结论莫西沙星能够有效改善肺炎支原体肺炎小鼠肺组织
Objective To investigate the protective effect of moxifloxacin(Moxi)on Mycoplasma pneumoniae pneumonia by downregulating long non-coding RNA growth inhibition specific gene(lncRNA GAS5)through non-receptor protein tyrosine kinase(JAK)/signaling and transcriptional activator protein(STAT)signaling pathways.Methods BA LB/c mice were randomly divided into control group,model group,experimental group and combined group.Mice in model group,experimental group and combined group were treated with nasal drops of Mycoplasma pneumoniae fluid to establish Mycoplasma pneumoniae pneumonia model.By intraperitoneal injection of the experimental group and the combined group of 100 mg·kg^(-1)moxifloxacin treatment;on the basis,the overexpressed plasmid pc-GAS5 was injected into the tail vein of the combined group.The control group and model group were injected with the same amount of 0.9%NaCl intraperitoneally.The serum levels of interleukin-1β(IL-1β)and IL-6 were detected by enzyme-linked immunosorbent assay(ELISA);the serum levels of superoxide dismutase(SOD)were detected by biochemical method;and the expression of IncRNA GAS5 in lung tissue was detected by realtime fluorescence quantitative polymerase chain reaction;the protein expression levels of B-cell lymphoma-2(Bcl-2),JAK2 and STAT3 in mouse lung were detected by Western blot.Results The serum IL-1βcontent in control group,model group and experimental group were(64.03±14.19),(254.85±63.79)and(157.46±42.93)pg·mL^(-1),respectively;IL-6 contents were(22.61±5.01),(184.14±39.50)and(132.64±25.47)pg·mL^(-1);SOD activities were(50.38±11.75),(28.17±6.71)and(37.67±8.09)U·mL^(-1),respectively;the relative expression levels of lncRNA GAS5 in lung tissues were 1.00±0.16,3.78±0.69 and 2.41±0.54,respectively.The relative expression levels of Bel-2 protein in control group,model group,experimental group and combined group were1.00±0.17,0.65±0.14,0.82±0.16 and 0.62±0.13,respectively;phospherylated JAK2/JAK2 ratios were1.00±0.20,4.98±1.01,2.13±0.52 and 4.21±0.92,
作者
郭晓华
祝伟
张倩
GUO Xiao-hua;ZHU Wei;ZHANG Qian(Department of Clinical Laboratory,The Fourth People's Hospital of Jinan,Jinan 250031,Shandong Province,China;Department of Respiratory Medicine,The Fourth People's Hospital of Jinan,Jinan 250031,Shandong Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2024年第19期2853-2857,共5页
The Chinese Journal of Clinical Pharmacology
