摘要
建立一测多评法同时测定甘青青兰中7个有效成分的含量,并对不同产地药材质量进行评价。采用HPLC,以乙腈-甲醇-0.2%磷酸水溶液作为流动相进行梯度洗脱,柱温35℃,进样量15μL,流速0.6 mL·min^(-1),检测波长215 nm。以迷迭香酸为内参物,计算其他6个成分的相对校正因子并计算含量,同时与外标法测定结果进行了对比。结果显示,青海省西宁市湟中县药材质量最优,其对羟基苯甲酸、大波斯菊苷、迷迭香酸、齐墩果酸和熊果酸质量分数均最高,分别为9.29、12.14、6.02、3.11、17.67 mg·g^(-1)。其次是西藏自治区昌都市察雅县药材,其白桦酯醇和白桦酯酸质量分数最高,分别为15.53、7.17 mg·g^(-1)。该方法结果准确、可靠、重复性好,适合于甘青青兰多成分同时测定,以含量为指标,不同产地样品有效成分含量存在显著性差异,可作为该药材质量评价的依据。
This study aims to establish a method for the simultaneous determination of 7 active components in Dracocephalum tanguticum and to evaluate the quality of medicinal materials from different habitats.The method was established with high performance liquid chromatography(HPLC)and the gradient elution was performed with the mobile phase of acetonitrile-methanol-0.2%phosphoric acid solution at a column temperature of 35℃,an injection volume of 15μL,and a flow rate of 0.6 mL·min^(-1).The detection wavelength was set as 215 nm.With rosmarinic acid as the internal reference,the relative correction factors and the content of other 6 components were calculated.The results were compared with those obtained with the external standard method.The results showed that the samples from Huangzhong county,Qinghai province had the best quality,with the highest content of p-hydroxybenzoic acid,cosmosiin,rosmarinic acid,oleanolic acid,and ursolic acid(9.29,12.14,6.02,3.11,17.67 mg·g^(-1)respectively).The samples from Chaya county,Tibet autonomous region ranked the second,with the highest content of betulin and betulinic acid(15.53,7.17 mg·g^(-1),respectively).The method is accurate,reliable,and repeatable and suitable for the simultaneous determination of multiple components in D.tanguticum.The content of functional components varied in the samples from different producing areas and can be used as the indicator for the quality evaluation of medicinal materials.
作者
叶菊
汪成
马钰洁
白玛扎西
唐媛媛
何旭光
马敏
YE Ju;WANG Cheng;MA Yu-jie;BAIMA Zha-xi;TANG Yuan-yuan;HE Xu-guang;MA Min(College of Pharmacy,Qinghai Minzu University,Xining 810007,China;Zunyi Medical and Pharmaceutical College,Zunyi 563001,China;Key Laboratory of Plant Resources of Qinghai-Tibet Plateau in Chemical Research,Xining 810007,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2023年第15期4253-4260,共8页
China Journal of Chinese Materia Medica
基金
青海省基础研究计划项目(2021-ZJ-707)
青海省自然科学基金项目(2022-ZJ-950Q)
青海民族大学高层次博士项目(2021XJG18)。
关键词
甘青青兰
不同产地
高效液相色谱法
校正因子
质量评价
Dracocephalum tanguticum
different producing areas
high performance liquid chromatography
correction factor
quality evaluation
