摘要
建立同时测定辣木叶中4个硫苷和5个黄酮类成分含量的一测多评法(QASM),并验证其可行性和适用性。采用Waters Acquity UPLC HSS T_(3)色谱柱(2.1 mm×100 mm,1.8μm),流动相为0.1%甲酸乙腈-0.3%甲酸水溶液,梯度洗脱,柱温40℃,流速0.4 mL·min^(-1),硫苷类成分检测波长225 nm,黄酮类成分检测波长260 nm。硫苷类和黄酮类成分分别以4-O-(α-L-鼠李糖基氧基)-苄基硫代葡萄糖苷和维采宁-2为内参物,分别计算4个硫苷和5个黄酮成分的相对校正因子,实现辣木叶中9个成分含量的一测多评。根据化合物的特点,采用内标法(ISM)建立了硫苷成分的含量测定方法,采用外标法(QSM)建立了黄酮成分的含量测定方法,验证一测多评法的准确性和可行性。t检验表明一测多评法所测9批辣木叶样品中硫苷成分的含量与内标法无显著性差异,所测样品中黄酮成分的含量与外标法无显著性差异。结果表明不同品种和不同产地辣木叶中硫苷和黄酮成分含量存在明显的差异,其中印度种中总硫苷和总黄酮含量最高,红河一号品种中含量最低。综上,一测多评法快速、简便、准确,可用于辣木叶中硫苷和黄酮类成分含量的同时测定,可为辣木叶的质量控制和开发利用提供实验数据。
To establish a method for simultaneously determining the content of four glucosinolates and five flavonoids in leaves of Moringa oleifera via quantitative analysis of multi-components by single-marker(QAMS)and verify the feasibility and applicability of the established method.The glucosinolates and flavonoids were analyzed via Waters Acquity UPLC HSS T_(3) column(2.1 mm×100 mm,1.8μm).The gradient elution was carried out with the mobile phase composed of 0.1%formic acid-acetonitrile and 0.3%formic acid at the flow rate of 0.4 mL·min^(-1)and the column temperature of 40℃.The wavelengths for the detection of glucosinolates and flavonoids were 225 nm and 260 nm,respectively.With 4-O-(α-L-rhamnoyloxy)-benzyl glucosinolate and vecenin-2 as internal reference substances,the relative correction factors of four glucosinolates and five flavonoids were respectively calculated for determining the content of the 9 ingredients in leaves of M.oleifera.To verify the accuracy and feasibility of QAMS,we used internal standard method(ISM)and external standard method(ESM)to determine the content of glucosinolates and flavonoids,respectively.The t-test results showed that there was no significant difference in the content of glucosinolates obtained by ISM and QAMS methods or in the content of flavonoids obtained by ESM and QAMS methods.The content of glucosinolates and flavonoids varied among M.oleifera of different varieties and from different producing areas.The total glucosinolates and total flavonoids had the highest content in the Indian variety while the lowest content in the variety‘Honghe No.1′.The established QAMS method is rapid,simple and accurate and can be used for simultaneous determination of glucosinolates and flavonoids in the leaves of M.oleifera.This study provides experimental data for the quality control and utilization of M.oleifera leaves.
作者
张明晓
李化
陈娜
向俊洁
林路洁
李志勇
杨滨
ZHANG Ming-xiao;LI Hua;CHEN Na;XIANG Jun-jie;LIN Lu-jie;LI Zhi-yong;YANG Bin(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Academician Workstation,Jiangxi University of Chinese Medicine,Nanchang 330004,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第12期3285-3294,共10页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2018YFC1706106)
中国中医科学院科技创新工程项目(CI2021A04511)。
关键词
辣木叶
一测多评法
内标法
外标法
硫代葡萄糖苷
黄酮
leaves of Moringa oleifera
quantitative analysis of multi-components by single-marker(QAMS)
internal standard method
external standard method
glucosinolates
flavonoids
