摘要
目的探讨新疆毛菊苣乙酸乙酯萃取物(CEE)对糖尿病小鼠的降糖作用。方法95%乙醇浸提得毛菊苣根部提取物;使用乙酸乙酯萃取得CEE;取昆明种小鼠,随机分为正常对照组、模型对照组、CEE小剂量组(150 g·kg^(-1))、CEE中剂量组(300 g·kg^(-1))、CEE大剂量组(600 g·kg^(-1))和二甲双胍组(300 mg·kg^(-1));正常对照组给予0.9%氯化钠溶液,其余各组腹腔注射四氧嘧啶200 mg·kg^(-1)建立小鼠糖尿病模型;记录小鼠体质量变化,以5 d为间隔测定血糖值;实验期末对小鼠进行糖耐量及胰岛素耐量实验;检测小鼠血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、胰岛素(INS)、丙二醛(MDA)、一氧化氮(NO)水平。结果小鼠造模成功率为88.57%,死亡率为11.43%。除正常对照组外,其余各组体质量均呈下降趋势,四氧嘧啶对小鼠体质量具有显著的影响;药物干预后,CEE对糖尿病小鼠体质量有明显的影响;根据糖耐量实验结果表明,CEE能明显降低小鼠血糖值(P<0.05或P<0.01),提高小鼠的糖耐量,并且大剂量组效果比中剂量组更佳;胰岛素耐量实验结果表明,大剂量CEE可明显增加小鼠对葡萄糖的利用能力,降低糖尿病小鼠的胰岛素耐量;血糖水平检测结果表明,CEE各剂量组可显著降低糖尿病小鼠血糖水平(P<0.05或P<0.01),呈剂量依赖性;血清测定结果表明,CEE各剂量组均能提高糖尿病小鼠血清中的INS和HDL-C的水平,降低糖尿病小鼠血清中的MDA、NO、TG、TC、LDL-C的水平,其中CEE大剂量组差异有统计学意义(P<0.05或P<0.01)。结论CEE对四氧嘧啶诱导的糖尿病小鼠有显著的降血糖作用,其机制可能是显著抑制α-葡萄糖苷酶的活性的同时调节糖脂代谢紊乱,增强机体抗氧化能力。
Objective To investigate the hypoglycemic effect of ethyl acetate extract of Cichorium glandulosum(CEE)on diabetic mice.Methods CEE was obtained from the root extract of Cichorium glandulosum by 95%ethanol extraction with ethyl acetate.Kunming mice were randomly divided into normal control group,model control group,CEE low-dose group(150 g·kg^(-1)),CEE medium-dose group(300 g·kg^(-1)),CEE high-dose group(600 g·kg^(-1))and metformin group(300 mg·kg^(-1)).Except for the normal control group,which was given 0.9%of sodium chloride solution,the other groups were intraperitoneally injected with alloxan to establish the mouse diabetes model.The changes in body mass of mice were recorded and the blood glucose level was measured at 5 d intervals.At the end of the experiment,glucose tolerance and insulin tolerance of mice were tested.Serum total cholesterol(TC),triacylglycerol(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),insulin(INS),malondialdehyde(MDA),and nitric oxide(NO)levels were measured in mice.by test kits.Results The success rate of mouse modeling was 88.57%,and the mortality rate was 11.43%.The body mass of all groups except the normal control group showed a decreasing trend and tetracosactide had a significant effect on the body mass of mice;Compared with the model control group,CEE had a significant effect on the body mass of diabetic mice after drug intervention.According to the results of glucose tolerance experiment,the blood glucose value of mice could be significantly reduced by CEE(P<0.05 or P<0.01).The results of insulin tolerance test showed that high dose CEE significantly increased the glucose utilization ability of mice and reduced the insulin tolerance of diabetes mice.The blood glucose level of diabetes mice significantly inhibited by CEE(P<0.05 or P<0.01).The levels of INS and HDL-C in the serum of diabetes mice could be increased by all CEE dose treatment groups,and the levels of MDA,NO,TG,TC,and LDL-C in the serum of diabetes mice were reduced.C
作者
苏林洁
邹楠
赫翠
陈卫军
秦冬梅
蔡钢
SU Linjie;ZOU Nan;HE Cui;CHEN Weijun;QIN Dongmei;CAI Gang(School of Pharmacy,Shihezi University/Key Laboratory of Xinjiang Botanical Medicine Resources Utilization,Ministry of Education,Shihezi 832000,China;The First Affiliated Hospital of Shihezi University,Shihezi 832000,China;College of Traditional Chinese Medicine,Xinjiang Second Medical College,Karamay 834000,China)
出处
《医药导报》
CAS
北大核心
2023年第10期1429-1436,共8页
Herald of Medicine
基金
国家自然科学基金资助项目(82160742)
兵团重点领域科技攻关项目(2023CB008-25)。
关键词
毛菊苣乙酸乙酯萃取物
四氧嘧啶
糖尿病
血糖
Ethyl acetate extract of Cichorium glandulosum
Alloxan
Diabetes
Blood glucose
