摘要
【目的】明确巨大普里斯特氏菌对不同黑叶白菜品种产量和品质的影响,为推动有益菌的应用提供理论依据。【方法】以海南当地主栽4个黑叶白菜品种为研究对象,设3个巨大普里斯特氏菌接种水平(3、6和9 kg/ha),以不接种为对照(CK),通过方差分析、主成分分析等方法分析巨大普里斯特氏菌对黑叶白菜产量及硝酸盐、游离氨基酸、可溶性蛋白、维生素C和可溶性糖含量的影响。【结果】随着巨大普里斯特氏菌接种量的增加,各品种黑叶白菜的硝酸盐含量均呈先下降后升高的变化趋势,最大降幅比CK降低72.50%;游离氨基酸和维生素C含量呈先升高后下降的变化趋势,最大增幅分别比CK增加79.13%和8.91%。8210号和A321的产量、甜脆331的可溶性蛋白含量、甜脆331和甜脆326的可溶性糖含量随着巨大普里斯特氏菌接种量的增加,整体上均呈先升高后下降的变化趋势,与CK相比,最大增加幅度分别为28.08%、5.25%和19.60%;而8210号的可溶性蛋白含量和A321的可溶性糖含量整体上呈先下降后升高的变化趋势,与CK相比,最大降低幅度分别为10.20%和10.42%。巨大普里斯特氏菌接种量和黑叶白菜品种2个因素在降低硝酸盐含量及提高游离氨基酸、维生素C和可溶性糖含量上具有极显著的交互效应(P<0.01);主成分分析发现,8210号和A321的最佳接种量为6 kg/ha,甜脆331和甜脆326的最佳接种量为3 kg/ha。【结论】接种巨大普里斯特氏菌在提高黑叶白菜产量的同时,能通过降低硝酸盐含量,提高游离氨基酸、维生素C和可溶性糖等途径改善其品质。此外,黑叶白菜品种显著影响巨大普里斯特氏菌在黑叶白菜产量和品质上的接种效应。
【Objective】The purpose of the study was to study the effects of Priestia megaterium on the yield and quali-ty of different varieties of black-leaf Chinese cabbage,and to provide theoretical basis for promoting the application of beneficial bacteria.【Method】Four black-leaf Chinese cabbage varieties were used,and three inoculation levels of P.megaterium(3,6 and 9 kg/ha)were set,with no inoculation as control(CK).Variance analysis and principal component analysis were used to analyze the effects of P.megaterium on the yield,nitrate content,free amino acid content,soluble protein content,vitamin C content and soluble sugar content of black-leaf Chinese cabbage.【Result】With the increase of P.megaterium inoculation amount,nitrate content of all varieties of black-leaf Chinese cabbage showed a trend of decreasing and then increasing,with the maximum decrease of 72.5%compared with CK.The free amino acid content and vitamin C content showed a trend of increasing and then decreasing,with the maximum increases of 79.13%and 8.91%respectively compared with CK.With the increase of P.megaterium inoculation amount,yield of No.8210 and A321,soluble protein content of Tiancui 331,soluble sugar content of Tiancui 331 and Tiancui 326 all showed an overall trend of increasing and then decreasing,with the maximum increases of 28.08%,5.25%and 19.60%respectively compared with CK.The soluble protein content in No.8210 and soluble sugar content in A321 showed an overall trend of decreasing and then increasing,with the maximum decreases of 10.20%and 10.42%respectively compared with CK.The two factors,P.megaterium inoculation amount and black-leaf Chinese cabbage variety,had extremely significant interac-tion effects on reducing nitrate content and increasing free amino acid content,vitamin C content and soluble sugar content(P<0.01).Principal component analysis revealed that the optimal inoculation amount for No.8210 and A321 Chinese cabbage was 6 kg/ha,and the amount for Tiancui 331 and Tiancui 326 was 3 kg/ha.【Conclusion】
作者
余小兰
李勤奋
李光义
邹雨坤
张俏燕
李晓亮
YU Xiao-lan;LI Qin-fen;LI Guang-yi;ZOU Yu-kun;ZHANG Qiao-yan;LI Xiao-liang(Environmental and Plant Protection Institute,ChineseAcademy ofTropicalAgricultural Sciences/Hainan DanzhouTropical Agro-ecosystem National Observation and Research Station/Key Laboratory of Low-carbon Green Agriculture in Tropical Region of China,Ministry of Agriculture and Rural Affairs,Haikou,Hainan 571101,China;Hainan Key Laboratory of Tropical Eco-circular Agriculture,Haikou,Hainan 571101,China;National Agricultural Experimental Station for Agricultural Environment/National Long-term Experimental Station for Agriculture Green Development,Danzhou,Hainan 571737,China;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Field Soil Scientific Research Station in Danzhou of Hainan Province,Danzhou,Hainan 571737,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2023年第5期1446-1454,共9页
Journal of Southern Agriculture
基金
海南省重点研发计划项目(ZDYF2022XDNY212)
海南省重大科技计划项目(ZDKJ2021009)
中国热带农业科学院基本科研业务费专项(1630042022010,1630042022002)。
关键词
巨大普里斯特氏菌
黑叶白菜
产量
品质
Priestia megaterium
black-leaf Chinese cabbage
yield
quality
