摘要
目的 通过对本地区急性呼吸道感染患儿进行病原体检测及实验室检查,探析联合检测病原体及炎性标志物在儿童急性呼吸道感染中的应用价值。方法 收集2018-2022年本院儿科接诊的1085例急性呼吸道感染患儿临床资料,采用间接免疫荧光法对呼吸道常见九种病原体抗体检测,包括五种呼吸道病毒(FIVA、FIVB、PIV、RSV、HADV)及四种非典型病原体(MP、CP、LP、QFR)。对经病原体IgM抗体检测结果为单一MP感染的患儿,采集其痰液标本进行细菌培养。采集患儿静脉血使用血液细胞自动分析仪检测白细胞计数(WBC)、血小板计数(PLT)水平,采用免疫比浊法检测血清C反应蛋白(CRP)水平。对比MP合并细菌感染与单一MP感染组患儿的炎性标志物水平。结果 1 085例急性呼吸道感染患儿中,316例血清病原体IgM抗体阳性,阳性率29.12%,其中192例患儿为单一病原体感染,124例患儿为两种及两种以上混合感染。单一病原体感染中,主要为MP感染(44.27%,85/192)及PIV感染(21.88%,42/192)。混合感染中,以两种病原体混合感染为主(91.13%,113/124),主要为MP+PIV混合感染(25.81%,32/124)。男性患儿中,血清病原体IgM抗体阳性率为25.93%,单一病原体感染阳性率为15.12%,单一MP感染阳性率为4.63%。女性患儿中,血清病原体IgM抗体阳性率33.18%,单一病原体感染阳性率为21.51%,两组患儿差异有统计学意义(P<0.05)。男性患儿中,单一PIV感染阳性率为3.09%,混合病原体感染阳性率为11.27%。女性患儿中,单一PIV感染阳性率为5.03%,混合病原体感染阳性率为11.67%,两组患儿对比差异无统计学意义(P>0.05)。对比不同年龄段患儿的病原体感染情况,婴儿组、幼儿组、学龄前组、学龄组患儿病原体阳性率及单一病原体感染阳性率差异有统计学意义(P<0.05),混合病原体感染阳性率、单一MP感染阳性率、单一PIV感染阳性率对比差异无统计学意义(P>0.05)。对比26例MP�
Objective By conducting pathogen testing and laboratory examinations on children with acute respiratory tract infections in the local area,the application value of combined detection of pathogens and inflammatory markers in children with acute respiratory tract infections was explored.Methods The clinical data of 1085children with acute re-spiratory infection admitted to the Department in our hospital from 2018to 2022were selected as the subjects of this stud-y.The antibodies against nine common respiratory pathogens in all children were detected by indirect immunofluorescence assay,including five respiratory viruses(FIVA,FIVB,PIV,RSV,HADV)and four atypical pathogens(MP,CP,LP,QFR).The sputum samples were collected for bacterial culture from children with a single MP infection detected by the pathogen IgM antibody test.The venous blood of the children was collected to detect the levels of white blood cell count(WBC)and platelet count(PLT)by the blood cell AutoAnalyzer,and the serum C-reactive protein(CRP)level was detected with the immune turbidimetry.The levels of inflammatory markers in children with MP combined bacterial infection and those with single MP infection were compared.Results Of 1085children with acute respiratory tract infection,316 were positive for serum pathogen IgM antibody,with a positive rate of 29.12%.Among them,192children were infected with a single pathogen,and 124children were infected with two or more kinds of mixed infections.Among single pathogen infections,the main ones were MP infection(44.27%,85/192)and PIV infection(21.88%,42/192).In mixed infections,the main infection was the combination of two pathogens(91.13%,113/124),and the main infection was the combination of MP+PIV(25.81%,32/124).In male children,the positive rate of serum pathogen IgM antibody was 25.93%,the pos-itive rate of single pathogen infection was 15.12%,and the positive rate of single MP infection was 4.63%.In female chil-dren,the positive rate of serum pathogen IgM antibody was 33.18%,and the positive rate of sin
作者
程瑞朵
李英
戴颖
汪奇伟
CHENG Ruiduo;DAI Ying;LI Ying;WANG Qiwei(Department of Pediatrics,Huaihe Hospital of Henan University,Kaifeng 475000,Henan,China)
出处
《中国病原生物学杂志》
CSCD
北大核心
2023年第10期1200-1204,共5页
Journal of Pathogen Biology
关键词
急性呼吸道感染
病原体
肺炎支原体
炎性标志物
acute respiratory infection
pathogens
Mycoplasma pneumoniae
inflammatory markers
